Cormack D
Exp Hematol. 1976 Sep;4(5):319-27.
The development of erythrocytic colonies in plasma cultures of C3H/Bi mouse fetal liver cells stimulated with erythropoietin was recorded by time-lapse cinemicrography, in order to investigate the nature of the colony-forming unit (CFU-E) from which they arose. Each colony was found to develop from a single cell, which in most cases underwent two divisions before acquiring hemoglobin, as detected by absorption in the Soret band. Less frequently, there were one or three divisions before hemoglobin appeared at 11.7 to 29.2 h. The diameter of the colony-forming cells in G2 was 16.0+/-1.3 mum (mean+/-SD). Daughter cells in each colony had similar generation times, with a TG of 11.0+/-2.2 h (mean+/-SD) for the first generation of colony cells. Evidence is presented that while some of the colony-forming cells were triggered into division by the erythropoietin added at plating, others had already been stimulated by endogenous erythropoietin in the fetus.
为了研究形成红细胞集落的集落形成单位（CFU-E）的性质，利用延时显微电影摄影技术记录了用促红细胞生成素刺激的C3H/Bi小鼠胎儿肝细胞血浆培养物中红细胞集落的发育情况。发现每个集落都由单个细胞发育而来，在大多数情况下，该细胞在获得血红蛋白（通过索雷特带吸收检测）之前经历两次分裂。在血红蛋白出现前11.7至29.2小时，较少情况下会有一次或三次分裂。处于G2期的集落形成细胞直径为16.0±1.3μm（平均值±标准差）。每个集落中的子细胞具有相似的世代时间，第一代集落细胞的TG为11.0±2.2小时（平均值±标准差）。有证据表明，虽然一些集落形成细胞在接种时被添加的促红细胞生成素触发分裂，但其他细胞已经受到胎儿内源性促红细胞生成素的刺激。
