Huysseune A, Van der heyden C, Sire J Y
Instituut voor Dierkunde, Universiteit Gent, Belgium.
Anat Embryol (Berl). 1998 Oct;198(4):289-305. doi: 10.1007/s004290050185.
In order to build a reference system to assess ongoing in vitro and in situ hybridisation experiments on epithelial-mesenchymal interactions governing odontogenesis in the zebrafish, we describe here the generation of the pharyngeal dentition, and the histological development of teeth up to fourteen days post-fertilization, using serial semithin sections, handmade and computer-assisted reconstructions and transmission electron microscopy. The tooth pattern in larval zebrafish is generated in a predictable, and bilaterally symmetrical manner from shortly before hatching onwards. Characteristics related to tooth development and structure differ considerably from those seen in juvenile specimens and those described for other bony fishes. Particular features related to the cyprinid condition include the complex epithelial connectivity and the mode of attachment of the teeth.
为了建立一个参考系统，以评估正在进行的关于斑马鱼牙发生过程中上皮-间充质相互作用的体外和原位杂交实验，我们在此描述了咽齿列的形成，以及受精后十四天内牙齿的组织学发育情况，采用了连续半薄切片、手工和计算机辅助重建以及透射电子显微镜技术。斑马鱼幼体的牙齿模式从孵化前不久开始以可预测的、双侧对称的方式形成。与牙齿发育和结构相关的特征与幼鱼标本中所见以及其他硬骨鱼类所描述的特征有很大不同。与鲤科鱼类情况相关的特殊特征包括复杂的上皮连接和牙齿的附着方式。
