Harrop J A, McDonnell P C, Brigham-Burke M, Lyn S D, Minton J, Tan K B, Dede K, Spampanato J, Silverman C, Hensley P, DiPrinzio R, Emery J G, Deen K, Eichman C, Chabot-Fletcher M, Truneh A, Young P R
Department of Molecular and Cellular Immunology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406, USA.
J Biol Chem. 1998 Oct 16;273(42):27548-56. doi: 10.1074/jbc.273.42.27548.
Herpesvirus entry mediator (HVEM), a member of the tumor necrosis factor (TNF) receptor family, mediates herpesvirus entry into cells during infection. Upon overexpression, HVEM activates NF-kappaB and AP-1 through a TNF receptor-associated factor (TRAF)-mediated mechanism. Using an HVEM-Fc fusion protein, we screened soluble forms of novel TNF-related proteins derived from an expressed sequence tag data base. One of these, which we designated HVEM-L, specifically bound to HVEM-Fc with an affinity of 44 nM. This association was confirmed with soluble and membrane forms of both receptor and ligand. HVEM-L mRNA is expressed in spleen, lymph nodes, macrophages, and T cells and encodes a 240-amino acid protein. A soluble, secreted form of the protein stimulates proliferation of T lymphocytes during allogeneic responses, inhibits HT-29 cell growth, and weakly stimulates NF-kappaB-dependent transcription.
疱疹病毒进入介质（HVEM）是肿瘤坏死因子（TNF）受体家族的成员，在感染期间介导疱疹病毒进入细胞。过表达时，HVEM通过肿瘤坏死因子受体相关因子（TRAF）介导的机制激活核因子κB（NF-κB）和活化蛋白-1（AP-1）。我们使用HVEM-Fc融合蛋白，从表达序列标签数据库中筛选新型TNF相关蛋白的可溶性形式。其中之一，我们将其命名为HVEM-L，以44 nM的亲和力特异性结合HVEM-Fc。受体和配体的可溶性及膜形式均证实了这种结合。HVEM-L mRNA在脾脏、淋巴结、巨噬细胞和T细胞中表达，编码一种240个氨基酸的蛋白质。该蛋白的可溶性分泌形式在同种异体反应期间刺激T淋巴细胞增殖，抑制HT-29细胞生长，并微弱刺激NF-κB依赖性转录。
