水稻矮缩植物呼肠孤病毒的P2蛋白是病毒吸附昆虫介体细胞所必需的。
The P2 protein of rice dwarf phytoreovirus is required for adsorption of the virus to cells of the insect vector.
作者信息
Omura T, Yan J, Zhong B, Wada M, Zhu Y, Tomaru M, Maruyama W, Kikuchi A, Watanabe Y, Kimura I, Hibino H
机构信息
National Agriculture Research Center, Tsukuba, Ibaraki 305, Japan.
出版信息
J Virol. 1998 Nov;72(11):9370-3. doi: 10.1128/JVI.72.11.9370-9373.1998.
Abstract
Intact particles of rice dwarf phytoreovirus adsorbed to and entered monolayer-cultured cells of the insect vector Nephotettix cincticeps and multiplied within the cells. Particles that lacked the P2 protein neither attached to nor infected such cells. Furthermore, P2-free particles obtained from a transmission-competent isolate of the virus were unable to infect insect vectors that had been allowed to feed on these virus particles through a membrane. However, when such virus particles were injected into insects via a glass capillary tube they successfully infected the insects, which became able to transmit the virus. These results support the hypothesis that, while P2-free particles can neither interact with nor infect cells in the intestinal tract of the insect vector, they do retain the ability to infect such cells when physically introduced into the hemolymph by injection.
摘要
水稻矮缩植物呼肠孤病毒的完整颗粒吸附并进入昆虫介体黑尾叶蝉的单层培养细胞，并在细胞内增殖。缺乏P2蛋白的颗粒既不附着也不感染此类细胞。此外，从具有传播能力的病毒分离株中获得的无P2颗粒无法感染通过膜取食这些病毒颗粒的昆虫介体。然而，当通过玻璃毛细管将此类病毒颗粒注射到昆虫体内时，它们成功地感染了昆虫，这些昆虫随后能够传播病毒。这些结果支持了这样一个假说，即虽然无P2颗粒既不能与昆虫介体肠道中的细胞相互作用也不能感染这些细胞，但当通过注射将它们物理引入血淋巴时，它们确实保留了感染此类细胞的能力。
相似文献
1
The P2 protein of rice dwarf phytoreovirus is required for adsorption of the virus to cells of the insect vector.水稻矮缩植物呼肠孤病毒的P2蛋白是病毒吸附昆虫介体细胞所必需的。
J Virol. 1998 Nov;72(11):9370-3. doi: 10.1128/JVI.72.11.9370-9373.1998.
2
The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus.外壳蛋白P2的缺失导致水稻矮缩植物呼肠孤病毒无法通过昆虫载体传播。
J Virol. 1997 Oct;71(10):8019-23. doi: 10.1128/JVI.71.10.8019-8023.1997.
3
Association of Rice gall dwarf virus with microtubules is necessary for viral release from cultured insect vector cells.水稻瘤矮病毒与微管的结合对于病毒从培养的昆虫载体细胞中释放是必要的。
J Virol. 2009 Oct;83(20):10830-5. doi: 10.1128/JVI.01067-09. Epub 2009 Jul 29.
4
Rice dwarf virus is engulfed into and released via vesicular compartments in cultured insect vector cells.水稻矮缩病毒通过培养的昆虫传毒介体细胞中的囊泡区室被摄入并释放。
J Gen Virol. 2008 Nov;89(Pt 11):2915-2920. doi: 10.1099/vir.0.2008/002063-0.
5
P2 protein encoded by genome segment S2 of rice dwarf phytoreovirus is essential for virus infection.水稻矮缩植物呼肠孤病毒基因组片段S2编码的P2蛋白对病毒感染至关重要。
Virology. 1996 Oct 15;224(2):539-41. doi: 10.1006/viro.1996.0560.
6
The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms.水稻矮缩病毒P2蛋白在体内与贝壳杉烯氧化酶相互作用，导致赤霉素生物合成减少并出现水稻矮缩症状。
Plant Physiol. 2005 Dec;139(4):1935-45. doi: 10.1104/pp.105.072306. Epub 2005 Nov 18.
7
Filamentous Structures Induced by a Phytoreovirus Mediate Viral Release from Salivary Glands in Its Insect Vector.一种植物呼肠孤病毒诱导的丝状结构介导病毒从其昆虫载体的唾液腺中释放。
J Virol. 2017 May 26;91(12). doi: 10.1128/JVI.00265-17. Print 2017 Jun 15.
8
Nonstructural protein Pns4 of rice dwarf virus is essential for viral infection in its insect vector.水稻矮缩病毒的非结构蛋白Pns4对其昆虫介体中的病毒感染至关重要。
Virol J. 2015 Dec 9;12:211. doi: 10.1186/s12985-015-0438-6.
9
Rice dwarf virus infection alters green rice leafhopper host preference and feeding behavior.水稻矮缩病毒感染改变了褐飞虱对水稻的取食偏好和行为。
PLoS One. 2018 Sep 7;13(9):e0203364. doi: 10.1371/journal.pone.0203364. eCollection 2018.
10
Electron microscopic imaging revealed the flexible filamentous structure of the cell attachment protein P2 of Rice dwarf virus located around the icosahedral 5-fold axes.电子显微镜成像显示，水稻矮缩病毒的细胞附着蛋白P2的柔性丝状结构位于二十面体的5次轴周围。
J Biochem. 2016 Feb;159(2):181-90. doi: 10.1093/jb/mvv092. Epub 2015 Sep 15.
引用本文的文献
1
A Review of Vector-Borne Rice Viruses.《媒介传播的水稻病毒综述》
Viruses. 2022 Oct 14;14(10):2258. doi: 10.3390/v14102258.
2
Roles of Bacterial Symbionts in Transmission of Plant Virus by Hemipteran Vectors.细菌共生体在半翅目介体传播植物病毒中的作用
Front Microbiol. 2022 Jan 27;13:805352. doi: 10.3389/fmicb.2022.805352. eCollection 2022.
3
Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial symbionts mediates their joint transovarial transmission.病毒病原体与昆虫共生细菌外膜上的孔蛋白通道的相互作用介导了它们的联合经卵传播。
Philos Trans R Soc Lond B Biol Sci. 2019 Mar 4;374(1767):20180320. doi: 10.1098/rstb.2018.0320.
4
Plant Virus-Insect Vector Interactions: Current and Potential Future Research Directions.植物病毒与昆虫载体的相互作用：当前及未来潜在的研究方向。
Viruses. 2016 Nov 9;8(11):303. doi: 10.3390/v8110303.
5
Electron microscopic imaging revealed the flexible filamentous structure of the cell attachment protein P2 of Rice dwarf virus located around the icosahedral 5-fold axes.电子显微镜成像显示，水稻矮缩病毒的细胞附着蛋白P2的柔性丝状结构位于二十面体的5次轴周围。
J Biochem. 2016 Feb;159(2):181-90. doi: 10.1093/jb/mvv092. Epub 2015 Sep 15.
6
Evolution of eukaryotic single-stranded DNA viruses of the Bidnaviridae family from genes of four other groups of widely different viruses.双顺反子病毒科真核单链DNA病毒由其他四类差异很大的病毒的基因进化而来。
Sci Rep. 2014 Jun 18;4:5347. doi: 10.1038/srep05347.
7
Life cycle of phytoreoviruses visualized by electron microscopy and tomography.电镜和断层扫描技术直观呈现植物呼肠孤病毒的生活周期。
Front Microbiol. 2013 Oct 16;4:306. doi: 10.3389/fmicb.2013.00306. eCollection 2013.
8
Rice gall dwarf virus exploits tubules to facilitate viral spread among cultured insect vector cells derived from leafhopper Recilia dorsalis.水稻瘿瘤矮缩病毒利用微管来促进其在由褐飞虱 Recilia dorsalis 衍生的培养昆虫载体细胞间的传播。
Front Microbiol. 2013 Jul 23;4:206. doi: 10.3389/fmicb.2013.00206. eCollection 2013.
9
Tubular structure induced by a plant virus facilitates viral spread in its vector insect.植物病毒诱导的管状结构有助于病毒在其介体昆虫中传播。
PLoS Pathog. 2012;8(11):e1003032. doi: 10.1371/journal.ppat.1003032. Epub 2012 Nov 15.
10
Rice dwarf viruses with dysfunctional genomes generated in plants are filtered out in vector insects: implications for the origin of the virus.在介体昆虫中，植物中产生的基因组功能失调的水稻矮化病毒被过滤掉：对病毒起源的影响。
J Virol. 2011 Mar;85(6):2975-9. doi: 10.1128/JVI.02147-10. Epub 2010 Dec 29.
本文引用的文献
1
Details of the arrangement of the outer capsid of rice dwarf phytoreovirus, as visualized by two-dimensional crystallography.通过二维晶体学观察到的水稻矮缩植物呼肠孤病毒外衣壳排列细节。
J Virol. 1997 Nov;71(11):8899-901. doi: 10.1128/JVI.71.11.8899-8901.1997.
2
The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus.外壳蛋白P2的缺失导致水稻矮缩植物呼肠孤病毒无法通过昆虫载体传播。
J Virol. 1997 Oct;71(10):8019-23. doi: 10.1128/JVI.71.10.8019-8023.1997.
3
P2 protein encoded by genome segment S2 of rice dwarf phytoreovirus is essential for virus infection.水稻矮缩植物呼肠孤病毒基因组片段S2编码的P2蛋白对病毒感染至关重要。
Virology. 1996 Oct 15;224(2):539-41. doi: 10.1006/viro.1996.0560.
4
Rescue of infectivity by sequential in vitro transcapsidation of rotavirus core particles with inner capsid and outer capsid proteins.通过用内衣壳蛋白和外衣壳蛋白对轮状病毒核心颗粒进行连续体外转衣壳来恢复感染性。
Virology. 1993 Jun;194(2):743-51. doi: 10.1006/viro.1993.1315.
5
A low-viscosity epoxy resin embedding medium for electron microscopy.一种用于电子显微镜的低粘度环氧树脂包埋介质。
J Ultrastruct Res. 1969 Jan;26(1):31-43. doi: 10.1016/s0022-5320(69)90033-1.
6
The outer capsid protein of rice dwarf virus is encoded by genome segment S8.水稻矮缩病毒的外壳蛋白由基因组片段S8编码。
J Gen Virol. 1989 Oct;70 ( Pt 10):2759-64. doi: 10.1099/0022-1317-70-10-2759.
Zotero 插件