发根农杆菌介导的自然遗传转化。两种二年生植物（比利时菊苣和胡萝卜）实现一年生开花。
Natural genetic transformation by agrobacterium rhizogenes . Annual flowering in two biennials, belgian endive and carrot.
作者信息
Limami MA, Sun LY, Douat C, Helgeson J, Tepfer D
机构信息
Institut National de la Recherche Agronomique, Federation Nationale de Producteurs d'Endive and Laboratoire du Metabolisme et de la Nutrition des Plantes, Institut National de la Recherche Agronomique, 78026, Versailles, cedex, France.
出版信息
Plant Physiol. 1998 Oct;118(2):543-50. doi: 10.1104/pp.118.2.543.
Abstract
Genetic transformation of Belgian endive (Cichorium intybus) and carrot (Daucus carota) by Agrobacterium rhizogenes resulted in a transformed phenotype, including annual flowering. Back-crossing of transformed (R1) endive plants produced a line that retained annual flowering in the absence of the other traits associated with A. rhizogenes transformation. Annualism was correlated with the segregation of a truncated transferred DNA (T-DNA) insertion. During vegetative growth, carbohydrate reserves accumulated normally in these annuals, and they were properly mobilized prior to anthesis. The effects of individual root-inducing left-hand T-DNA genes on flowering were tested in carrot, in which rolC (root locus) was the primary promoter of annualism and rolD caused extreme dwarfism. We discuss the possible adaptive significance of this attenuation of the phenotypic effects of root-inducing left-hand T-DNA.
摘要
发根农杆菌对比利时菊苣（菊苣）和胡萝卜（胡萝卜）的遗传转化产生了一种转化表型，包括一年生开花。对转化的（R1）菊苣植株进行回交，得到了一个品系，该品系在没有与发根农杆菌转化相关的其他性状的情况下仍保持一年生开花。一年生特性与截短的转移DNA（T-DNA）插入的分离相关。在营养生长期间，碳水化合物储备在这些一年生植物中正常积累，并在开花前被适当调动。在胡萝卜中测试了单个诱导根的左手T-DNA基因对开花的影响，其中rolC（根位点）是一年生特性的主要启动子，rolD导致极端矮化。我们讨论了这种诱导根的左手T-DNA表型效应减弱的可能适应性意义。
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