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[运用放射免疫法对大肠杆菌和枯草芽孢杆菌诱导系统的定量分析]

[Quantitative analysis of inducible systems of Escherichia coli and Bacillus subtilis using radioimmunological methods].

作者信息

Suslov A V, Suslova I N

机构信息

B.P. Konstantinov St.-Petersburg Institute of Nuclear Physics, Academy of Sciences of Russia, Gatchina.

出版信息

Radiats Biol Radioecol. 1998 Jul-Aug;38(4):495-501.

PMID:9765667
Abstract

By RIA dot-blot method two main cell system, system SOS-repair and heat shock system, was showed possibility to receive as well qualitative as quantitative results influence of different induced agents. Was showed quantitative differences in initial levels enzymes of RecA and CroEL in different strains of E. coli, as well of principle possibility to using received antibody, which has high specific to this enzymes, for investigation reactions this systems in the other bacterial species, such as Bac. subtilis.

摘要

通过放射免疫斑点印迹法表明,两个主要细胞系统,即SOS修复系统和热休克系统,有可能获得不同诱导剂影响的定性和定量结果。结果显示,不同大肠杆菌菌株中RecA和CroEL酶的初始水平存在定量差异,同时也表明使用对这些酶具有高特异性的抗体来研究其他细菌物种(如枯草芽孢杆菌)中这些系统的反应具有基本可能性。

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