Neurogenesis and migration of receptor neurons in the vomeronasal sensory epithelium in the opossum, Monodelphis domestica.

The sensory epithelium of the vomeronasal organ (VNO) contains primary chemosensory receptor neurons that project to the accessory olfactory bulb (AOB). In the present study, neurogenesis and cell migration in the sensory epithelium of the VNO were analyzed in opossums (Monodelphis domestica) by using bromodeoxyuridine (BrdU) labeling. 1) In the VNO of normal adult opossums, BrdU labeled a small number of cells localized in the basal region of the sensory epithelium. After 1 or 2 weeks of survival, the labeled cells appeared in the receptor cell layers and became receptor neurons, as indicated by coexpression of the G proteins G(i alpha2) or G(o alpha). 2) In the VNO in which the receptor neurons had been destroyed by removing the AOB, the number of BrdU-labeled cells in the reconstituting sensory epithelium was greatly increased compared with that in the intact VNO. The labeled cells were also located in the basal region of the sensory epithelium. 3) In the developing VNO (at postnatal day 10), more cells in the basal region of the sensory epithelium were labeled than in the adult VNO, indicating rapid cell proliferation; and there appeared to be more labeled cells in the basal region near the margins of the sensory epithelium where it meets the nonsensory epithelium. These observations demonstrate that, in the opossum VNO, there is a population of proliferating cells in the basal region close to the basal lamina in the sensory epithelium. The newly generated neurons in the basal region migrate vertically into the receptor cell layer.