Parnaby-Price A, Stanford M R, Biggerstaff J, Howe L, Whiston R A, Marshall J, Wallace G R
Department of Ophthalmology, St. Thomas' Hospital, London, United Kingdom.
J Leukoc Biol. 1998 Oct;64(4):434-40. doi: 10.1002/jlb.64.4.434.
Leukocyte trafficking from blood into tissue is a fundamental process in immune surveillance and the immune response to stimuli. Experimental autoimmune uveitis (EAU) is an animal model for posterior uveitis and is mediated by T lymphocytes and macrophages that infiltrate the posterior segment of the eye. To analyze leukocyte migration into retinal tissue during the course of EAU, labeled cells were identified in vivo by scanning laser ophthalmoscopy and in retinal flatmounts by confocal microscopy. Adhesion of blood leukocytes to retinal endothelial cells in vivo was significantly raised 48 h before the appearance of clinical disease, and this correlated with the increased expression of CD54 on retinal vessels. Mitogen-activated spleen cells and CD4+ T cells only entered into retinal tissue in animals with clinical disease and not naive recipients. The disease status of the donor animal had no effect on leukocyte trafficking. These results, which identify leukocyte-endothelial cell interactions in vivo, suggest that the activation of the retinal endothelium is a prerequisite to leukocyte adhesion and extravasation into ocular tissue during EAU.
白细胞从血液进入组织是免疫监视以及对刺激产生免疫反应的一个基本过程。实验性自身免疫性葡萄膜炎（EAU）是后葡萄膜炎的一种动物模型，由浸润眼后段的T淋巴细胞和巨噬细胞介导。为了分析EAU病程中白细胞向视网膜组织的迁移，通过扫描激光检眼镜在体内以及通过共聚焦显微镜在视网膜铺片中识别标记细胞。在临床疾病出现前48小时，体内血白细胞与视网膜内皮细胞的黏附显著增加，这与视网膜血管上CD54表达的增加相关。丝裂原激活的脾细胞和CD4+ T细胞仅在患有临床疾病的动物中进入视网膜组织，而在未感染的受体中则不会。供体动物的疾病状态对白细胞迁移没有影响。这些在体内确定白细胞-内皮细胞相互作用的结果表明，视网膜内皮细胞的激活是EAU期间白细胞黏附并渗出到眼组织的先决条件。
