Ricard-Blum S, Bresson-Hadni S, Grenard P, Humbert P, Carbillet J P, Risteli L, Vuitton D A
Institut Pasteur de Lyon, France.
Parasitol Res. 1998 Sep;84(9):715-9. doi: 10.1007/s004360050475.
Cutaneous parasitic lesions, associated with a dense fibrous reaction, markedly improved under albendazole treatment in one case of supraumbilical skin localization of alveolar echinococcosis. Since collagen cross-linking increases during fibrogenesis and contributes to the stability of fibrotic lesions, we monitored the level of the cross-links pyridinoline and pentosidine in skin lesions from this patient to determine if they would reflect the changes occurring during treatment. We looked at the deposition of cross-linked type I collagen by immunohistochemistry and also measured the serum concentrations of pentosidine and of a fragment of type I collagen (ICTP), which contains a site of pyridinoline formation. Albendazole treatment did not affect either the collagen content of skin lesions or the serum concentrations of ICTP and pentosidine, but it led to a pronounced decrease in pyridinoline level concomitant with the disappearance, observed by immunohistochemistry, of extensively cross-linked fibrotic type I collagen. The follow-up of collagen cross-linking by pyridinoline in skin tissue thus appears to be useful in reflecting the improvement of fibrotic skin diseases during therapy.
在一例脐上皮肤定位的肺泡型棘球蚴病患者中，伴有致密纤维反应的皮肤寄生虫病变在阿苯达唑治疗下明显改善。由于在纤维化过程中胶原交联增加并有助于纤维化病变的稳定性，我们监测了该患者皮肤病变中交联吡啶啉和戊糖苷的水平，以确定它们是否会反映治疗期间发生的变化。我们通过免疫组织化学观察了交联I型胶原的沉积情况，还测量了血清中戊糖苷和I型胶原片段（ICTP）的浓度，ICTP含有吡啶啉形成位点。阿苯达唑治疗既不影响皮肤病变的胶原含量，也不影响ICTP和戊糖苷的血清浓度，但通过免疫组织化学观察发现，它导致吡啶啉水平显著降低，同时广泛交联的纤维化I型胶原消失。因此，通过吡啶啉对皮肤组织中的胶原交联进行随访似乎有助于反映治疗期间纤维化皮肤病的改善情况。
