Levy R, Dilley J
Proc Natl Acad Sci U S A. 1978 May;75(5):2411-5. doi: 10.1073/pnas.75.5.2411.
B leukemia cells from four different patients were hybridized with a mouse myeloma cell line with polyethylene glycol as a fusing agent. The original leukemia cells all expressed immunoglobulin on their surface, but failed to secrete it. Over 200 different human-mouse somatic cell hybrids were obtained; 57% of them secreted human immunoglobulin in large amounts. Human immunoglobulin secretion can be a stable property of these hybrid cells over months of continuous culture. In each case the human immunoglobulin secreted was restricted to the light chain type expressed by the parental B leukemia cell. In addition, these hybrid cells secreted the original mouse myeloma protein and a variety of mixed human-mouse immunoglobulin molecules.
来自四名不同患者的B淋巴细胞白血病细胞与一种小鼠骨髓瘤细胞系进行杂交，使用聚乙二醇作为融合剂。原始的白血病细胞在其表面均表达免疫球蛋白，但无法分泌它。获得了200多种不同的人-鼠体细胞杂种；其中57%大量分泌人免疫球蛋白。人免疫球蛋白的分泌在连续培养数月后可以是这些杂种细胞的稳定特性。在每种情况下，分泌的人免疫球蛋白都局限于亲代B淋巴细胞白血病细胞所表达的轻链类型。此外，这些杂种细胞分泌原始的小鼠骨髓瘤蛋白以及多种人-鼠混合免疫球蛋白分子。
