Tada N, Hämmerling U
Immunogenetics. 1980 Jul;11(1):7-19. doi: 10.1007/BF01567765.
The 1.29 cell line is a nonsecreting B-cell leukemia which bears two different immunoglobulin isotypes on its surface, IgM and IgX. The 1.29 cells were hybridized with nonsecreting myeloma cells giving rise to dozens of immunoglobulin secreting hybridomas. These fall into three groups differing in the class of immunoglobulin they secrete. Cells of the first group secrete pentameric IgM (mu, kappa), those of the second group secrete an unknown immunoglobulin, IgX, which may constitute an allotype of IgA, and those of the third group produce light chains only. The two complete immunoglobulins, IgM and IgX, have the same idiotype, as revealed by serological cross-reactivity of an exhaustively absorbed rabbit anti-idiotype serum. The molecular sizes of the heavy chains of the secreted IgM and IgX are slightly smaller than the mu and delta chains, respectively, which are derived from the surface of normal B cells as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
1.29细胞系是一种非分泌型B细胞白血病，其表面带有两种不同的免疫球蛋白同种型，即IgM和IgX。1.29细胞与非分泌型骨髓瘤细胞杂交，产生了数十种分泌免疫球蛋白的杂交瘤。这些杂交瘤分为三组，它们分泌的免疫球蛋白类别不同。第一组细胞分泌五聚体IgM（μ，κ），第二组细胞分泌一种未知的免疫球蛋白IgX，它可能构成IgA的一种同种异型，第三组细胞仅产生轻链。通过彻底吸收的兔抗独特型血清的血清学交叉反应表明，两种完整的免疫球蛋白IgM和IgX具有相同的独特型。根据十二烷基硫酸钠-聚丙烯酰胺凝胶电泳判断，分泌的IgM和IgX重链的分子大小分别略小于源自正常B细胞表面的μ链和δ链。
