Yarmush M L, Gates F T, Weisfogel D R, Kindt T J
Proc Natl Acad Sci U S A. 1980 May;77(5):2899-903. doi: 10.1073/pnas.77.5.2899.
Standard cell-fusion techniques have been used to generate hybrid cells from rabbit spleen cells and mouse myeloma cell lines. The hybrids were selected for secretion of rabbit immunoglobulin. Detailed allotype analyses were carried out for 189 cell lines found to be immunoglobulin positive: 1 produced an intact immunoglobulin molecule with antibody activity, 143 produced rabbit light (L) chains, 36 produced rabbit heavy (H) chains, and 9 cell lines gave negative results in tests for group a and b allotypes. Fusions with a nonproducing murine myeloma cell line (SP-2) yielded only L chain-secreting hybrids, whereas 27% of hybrids resulting from fusion with an L chain-producing line (PU) secreted rabbit H chains paired with mouse L chains. Several of the hybridomas have maintained the ability to produce rabbit immunoglobulin chains in culture for almost 1 year and can be propagated as ascites tumors in athymic (nude) mice. Cytogenetic analyses have detected no rabbit chromosomes in the stable hybrid cell lines.
标准的细胞融合技术已被用于从兔脾细胞和小鼠骨髓瘤细胞系中产生杂交细胞。选择分泌兔免疫球蛋白的杂交细胞。对189个免疫球蛋白呈阳性的细胞系进行了详细的同种异型分析:1个产生具有抗体活性的完整免疫球蛋白分子,143个产生兔轻链(L链),36个产生兔重链(H链),9个细胞系在a和b组同种异型检测中呈阴性结果。与不产生抗体的小鼠骨髓瘤细胞系(SP-2)融合仅产生分泌L链的杂交细胞,而与产生L链的细胞系(PU)融合产生的杂交细胞中有27%分泌与小鼠L链配对的兔H链。一些杂交瘤在培养中保持产生兔免疫球蛋白链的能力近1年,并且可以作为腹水瘤在无胸腺(裸)小鼠中繁殖。细胞遗传学分析在稳定的杂交细胞系中未检测到兔染色体。