Toama M A, Issa A A, Ashour M S
Pharmazie. 1978 Feb-Mar;33(2-3):100-2.
The factors affecting the sensitivity of nafcillin, vancomycin, oleandomycin, chloramphenicol, methacycline and novobiocin assay, using the paper disc diffusion method, were studied. Agar concentration and pour volume were found to influence the assay sensitivity of antibiotics. The type of assay medium was effective e.g. brain heart infusion agar was the best medium for nafcillin and methacycline; synthetic amino acid medium for oleandomycin; Muller Hinton agar for vancomycin and chloramphenicol; and G & R medium for novobiovicin. All additives, including inorganic ions, sugar, organic acids, complexing agents, surface active agents and blood, decreased the activities of the studied antibiotics. Higher activities of vancomycin, methacycline were observed at pH = 5, of nafcillin, chloramphenicol, and novobiocin at pH = 6, and of oleandomycin at pH = 7.
采用纸片扩散法，研究了影响萘夫西林、万古霉素、竹桃霉素、氯霉素、甲烯土霉素和新生霉素检测灵敏度的因素。发现琼脂浓度和倾注量会影响抗生素的检测灵敏度。检测培养基的类型有影响，例如脑心浸液琼脂是萘夫西林和甲烯土霉素的最佳培养基；合成氨基酸培养基用于竹桃霉素；Muller Hinton琼脂用于万古霉素和氯霉素；G&R培养基用于新生霉素。所有添加剂，包括无机离子、糖、有机酸、络合剂、表面活性剂和血液，都会降低所研究抗生素的活性。在pH = 5时观察到万古霉素、甲烯土霉素活性较高，在pH = 6时萘夫西林、氯霉素和新生霉素活性较高，在pH = 7时竹桃霉素活性较高。
