Morier Díaz L, Alemán Miranda M R, Castillo Alvarez A, Pérez Forcada V
Instituto de Medicina Tropical Pedro Kouri.
Rev Cubana Med Trop. 1991;43(3):156-61.
We study a new arthropod cell line, AP-64, which was obtained from Aedes pseudoscutellaris larvae. The best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. The immunofluorescence technique is applied to detect early multiplication of those viruses.