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[Preliminary study of AP-64 cell line (Aedes pseudoscutellaris) for dengue-1 and -2 virus multiplication].

作者信息

Morier Díaz L, Alemán Miranda M R, Castillo Alvarez A, Pérez Forcada V

机构信息

Instituto de Medicina Tropical Pedro Kouri.

出版信息

Rev Cubana Med Trop. 1991;43(3):156-61.

PMID:9768180
Abstract

We study a new arthropod cell line, AP-64, which was obtained from Aedes pseudoscutellaris larvae. The best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. The immunofluorescence technique is applied to detect early multiplication of those viruses.

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