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[用于诊断人类钩端螺旋体病的血清学技术的比较研究]

[Comparative study of serologic techniques for the diagnosis of human leptospirosis].

作者信息

Regalado Seguí D, López Acosta C, Obregón Fuentes A M

机构信息

Instituto de Medicina Tropical Pedro Kouri.

出版信息

Rev Cubana Med Trop. 1992;44(2):129-33.

PMID:9768203
Abstract

A comparative study was carried out in order to assess 3 serological techniques employed at the Provincial Centers of Hygiene and Epidemiology for diagnosing leptospirosis. The study included 49 sera from patients with leptospirosis confirmed by the techniques of passive hemoagglutination (HA), plate macroscopic agglutination with thermoresistant antigen (TR) and indirect immunofluorescence (IIF). Sensitivity was 90% for HA, 96% for TR and 84% for IIF. IIF was performed with the use of 5 different antigens and the highest serological reactivity was obtained with the strain belonging to Canicola serogroup.

摘要

为评估省卫生与流行病学中心用于诊断钩端螺旋体病的3种血清学技术,开展了一项对比研究。该研究纳入了49份血清,这些血清来自经被动血凝试验(HA)、耐热抗原平板宏观凝集试验(TR)和间接免疫荧光试验(IIF)技术确诊为钩端螺旋体病的患者。HA的灵敏度为90%,TR为96%,IIF为84%。IIF使用5种不同抗原进行,用犬型血清群菌株获得了最高的血清学反应性。

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