Martínez Machín G, Alvarez Bernal L P, Fernández Andreu C, Llop Hernández A
Instituto de Medicina Tropical Pedro Kouri Laboratorio de Micologia.
Rev Cubana Med Trop. 1992;44(2):141-4.
The tube agglutination (TA) technique was normalized in order to determine anti-Cryptococcus neoformans antibodies, according to Palmer et al., by employing positive and negative control sera and an antigenic suspension prepared from an autochtonous strain of Cryptococcus neoformans varneofornnans. Three groups of human sera were studied and the role of TA in detecting antibodies in the group of sera from patients with active cryptococcosis (100%) was shown. In sera from banks positiveness was only 6%, while in the group of fowl breeders, considered to be "exposed", positiveness was found to be 16%, although with low titres. A discussion is carried out about the value of this technique as a complement in the diagnosis and prognosis of cryptococcosis.
为了检测抗新型隐球菌抗体，根据帕尔默等人的方法，采用阳性和阴性对照血清以及由一株新型隐球菌变种新形隐球菌的本地菌株制备的抗原悬液，对试管凝集（TA）技术进行了标准化。研究了三组人血清，并显示了TA在检测活动性隐球菌病患者血清组中抗体的作用（100%）。在血库血清中阳性率仅为6%，而在被认为“暴露”的家禽饲养者组中，阳性率为16%，尽管滴度较低。文中对该技术作为隐球菌病诊断和预后补充手段的价值进行了讨论。
