Illnait Zaragozí M T, Martínez Machín G, Fernández Andreu C
Instituto de Medicina Tropical Pedro Kourí.
Rev Cubana Med Trop. 1998;50(1):27-30.
Two immunization schemes were applied to rabbits with an autoctonous strain aimed at obtaining an anti-Crytococcus neoformans IgG. Whole cells treated with formalin were used. Dosages and immunization routes were different. The sera obtained were titered and that with the highest titer was selected (1:1 024 by the laminar agglutination method) and purified by saline precipitation and ion exchange chromatography. An IgG of high purity and biological activity was obtained.
将两种免疫方案应用于携带自体菌株的兔子，旨在获得抗新型隐球菌IgG。使用经福尔马林处理的全细胞。剂量和免疫途径不同。对获得的血清进行滴定，选择滴度最高的血清（通过平板凝集法为1:1024），并通过盐沉淀和离子交换色谱法进行纯化。获得了高纯度和生物活性的IgG。
