[一种用于检测甲型肝炎病毒IgM抗体的酶联免疫吸附测定法的开发与评估]
[Development and evaluation of an ELISA for the detection of IgM antibodies against the hepatitis A virus].
作者信息
Rodríguez Lay L, Más Lago P, Balmaseda A, Comellas M M, Delgado G, Palomera R
机构信息
Instituto de Medicina Tropical Pedro Kouri.
出版信息
Rev Cubana Med Trop. 1994;46(2):86-9.
We present the results of the normalization of an IgM capture ELISA method for the diagnosis of type A viral hepatitis with reagents produced in the laboratory and its comparison with the "Diag-A-Hep" commercial ELISA. The results attained were: sensibility by 91%; specificity by 100%; and coincidence of the two systems by 97%. Results are discussed and their relationship with clinical symptoms and epidemiological characteristics is established. The results attained in 13 serum samples taken from patients seen during 2 acute viral hepatitis outbreaks agreed with clinical findings.
我们展示了使用实验室自制试剂对用于诊断甲型病毒性肝炎的IgM捕获ELISA方法进行标准化的结果，并将其与“Diag-A-Hep”商用ELISA进行比较。获得的结果为：敏感性91%；特异性100%；两个系统的符合率97%。对结果进行了讨论，并确定了它们与临床症状和流行病学特征的关系。从2次急性病毒性肝炎暴发期间就诊的患者采集的13份血清样本所获结果与临床发现相符。
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