[一种用于检测甲型肝炎病毒IgM抗体的酶联免疫吸附测定法的开发与评估]

[Development and evaluation of an ELISA for the detection of IgM antibodies against the hepatitis A virus].

作者信息

Rodríguez Lay L, Más Lago P, Balmaseda A, Comellas M M, Delgado G, Palomera R

机构信息

Instituto de Medicina Tropical Pedro Kouri.

出版信息

Rev Cubana Med Trop. 1994;46(2):86-9.

Abstract

We present the results of the normalization of an IgM capture ELISA method for the diagnosis of type A viral hepatitis with reagents produced in the laboratory and its comparison with the "Diag-A-Hep" commercial ELISA. The results attained were: sensibility by 91%; specificity by 100%; and coincidence of the two systems by 97%. Results are discussed and their relationship with clinical symptoms and epidemiological characteristics is established. The results attained in 13 serum samples taken from patients seen during 2 acute viral hepatitis outbreaks agreed with clinical findings.

摘要

我们展示了使用实验室自制试剂对用于诊断甲型病毒性肝炎的IgM捕获ELISA方法进行标准化的结果，并将其与“Diag-A-Hep”商用ELISA进行比较。获得的结果为：敏感性91%；特异性100%；两个系统的符合率97%。对结果进行了讨论，并确定了它们与临床症状和流行病学特征的关系。从2次急性病毒性肝炎暴发期间就诊的患者采集的13份血清样本所获结果与临床发现相符。

相似文献

1

[Development and evaluation of an ELISA for the detection of IgM antibodies against the hepatitis A virus].[一种用于检测甲型肝炎病毒IgM抗体的酶联免疫吸附测定法的开发与评估]

Rev Cubana Med Trop. 1994;46(2):86-9.

2

An in-house-anti-hepatitis A virus (HAV)-specific immunoglobulin M capture enzyme-linked immunosorbent assay: evaluation and application to an HAV outbreak.一种内部抗甲型肝炎病毒（HAV）特异性免疫球蛋白M捕获酶联免疫吸附测定法：评估及其在甲型肝炎病毒暴发中的应用

J Med Virol. 2009 Sep;81(9):1513-6. doi: 10.1002/jmv.21578.

3

[Detection of immunoglobulin A in serum and saliva of patients with hepatitis A].[甲型肝炎患者血清和唾液中免疫球蛋白A的检测]

Rev Cubana Med Trop. 1994;46(1):16-9.

4

[Filter paper sampling for the detection of anti-hepatitis A IgM antibodies].[用于检测抗甲型肝炎IgM抗体的滤纸采样]

Rev Cubana Med Trop. 1998;50(1):42-7.

5

Evaluation of human (genotype 1) and swine (genotype 4)-ORF2-based ELISAs for anti-HEV IgM and IgG detection in an endemic country and search for type 4 human HEV infections.在一个地方流行国家评估基于人（1型基因型）和猪（4型基因型）开放阅读框2（ORF2）的酶联免疫吸附测定（ELISA）检测抗戊型肝炎病毒（HEV）IgM和IgG，并寻找4型人HEV感染情况。

J Viral Hepat. 2007 Jun;14(6):435-45. doi: 10.1111/j.1365-2893.2006.00801.x.

6

Indigenous anti-hepatitis A virus IgM capture ELISA for the diagnosis of hepatitis A.用于甲型肝炎诊断的本土抗甲型肝炎病毒 IgM 捕获酶联免疫吸附测定法

Indian J Med Res. 1994 Jun;99:243-51.

7

[Detection of IgM-antibody to hepatitis G virus and its clinical significance].庚型肝炎病毒IgM抗体的检测及其临床意义

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1999 Mar;13(1):77-9.

8

[A comparison of the detection of IgM antibodies against the viral capsid antigen (VCA) of EBV (Epstein-Barr virus) in various groups of patients, using indirect immunofluorescence, indirect ELISA and reverse ELISA. Study of the diagnostic efficacy of the anti-VCA EBV IgM ELISA-VIDITEST kit].[采用间接免疫荧光法、间接酶联免疫吸附测定法和反向酶联免疫吸附测定法对不同患者组中针对EB病毒（Epstein-Barr病毒）病毒衣壳抗原（VCA）的IgM抗体进行检测的比较。抗VCA EBV IgM酶联免疫吸附测定试剂盒（ELISA-VIDITEST）的诊断效能研究]

Klin Mikrobiol Infekc Lek. 2004 Aug;10(4):186-90.

9

Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections.用于诊断登革热感染的免疫球蛋白M和G捕获酶联免疫吸附测定Panbio试剂盒的评估。

J Clin Virol. 2007 Jul;39(3):194-8. doi: 10.1016/j.jcv.2007.04.003. Epub 2007 May 22.

10

[Comparison of the reliability of two ELISA kits for detecting IgM antibody against hepatitis E virus].[两种用于检测戊型肝炎病毒IgM抗体的酶联免疫吸附测定试剂盒可靠性的比较]

Zhonghua Yu Fang Yi Xue Za Zhi. 2008 Sep;42(9):667-71.

引用本文的文献

1

Anti-hepatitis A virus immunoglobulin M antibodies in urine samples for rapid diagnosis of outbreaks.尿液样本中的抗甲型肝炎病毒免疫球蛋白M抗体用于暴发疫情的快速诊断。

Clin Diagn Lab Immunol. 2003 May;10(3):492-4. doi: 10.1128/cdli.10.3.492-494.2003.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。