Castillo Alvarez A, Morier Díaz L
Instituto de Medicina Tropical Pedro Kouri.
Rev Cubana Med Trop. 1994;46(3):139-43.
Using the C6/36 clone of the Aedes albopictus cell line, a derived cell line was obtained capable of growing in a commercial serum-free medium facilitating this line to keep its morphologic characteristics and adhesion capacity. The growth speed rate reduction of the adapted cell line was compensated by its low maintenance cost. Its usefulness in the multiplication of dengue viruses 1 and 2 was tested; the reported line showed the same susceptibility for the 2 agents than the original cell line when the indirect fluorescence technique was applied.
使用白纹伊蚊细胞系的C6/36克隆,获得了一种能够在商业无血清培养基中生长的衍生细胞系,这有助于该细胞系保持其形态特征和黏附能力。适应后的细胞系生长速度降低,但因其维护成本低而得到弥补。测试了其在登革病毒1型和2型增殖中的效用;当应用间接荧光技术时,该报告的细胞系对这两种病原体的易感性与原始细胞系相同。