[用于检测人细小病毒B19抗体的超微量酶联免疫吸附测定法的标准化]
[Normalization of an ultramicroELISA for the detection of antibodies against human Parvovirus B19].
作者信息
Morier Díaz L, Castillo Alvarez A, Laferté Serrano J
机构信息
Instituto de Medicina Tropical Pedro Kouri.
出版信息
Rev Cubana Med Trop. 1994;46(3):183-4.
PMID:9768261
Abstract
An ultramicroELISA (UME) method was normalized for the detection of antibodies to serum human parvovirus B19. The optimum antigen concentration determined was 400 ng/ml, serum dilution was of 1:100; and the conjugate work dilution was of 1:2,000. 11 paired serum samples were also evaluated and antibodies were detected. The usefulness of the analyzed system is discussed.
摘要
一种超微量酶联免疫吸附测定法（UME）被标准化用于检测血清中人类细小病毒B19的抗体。确定的最佳抗原浓度为400 ng/ml，血清稀释度为1:100；结合物工作稀释度为1:2000。还对11对血清样本进行了评估并检测到了抗体。讨论了所分析系统的实用性。
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