[Indirect ultramicroELISA for the detection of total adenovirus antibodies in human serum].

An indirect ultramicroELISA method was normalized for the detection of total antibodies to Adenovirus (AD) (UMELISA AD). The optimum concentration of surface antigen determined was 30 micrograms/mL; serum dilution was of 1:40; and conjugate dilution, of 1:1,000. The UMELISA AD was compared with the complement fixing and indirect immunofluorescence techniques. Results showed accordance by 88% and 96.6%, respectively. The method is valid for the screening of antibodies in seroepidemiological studies as well as for the diagnosis of the infection by paired serum samples.