[间接超微量酶联免疫吸附测定法检测人血中抗巨细胞病毒总抗体]
[Indirect ultramicroElisa for the detection of total antibodies against cytomegaloviruses in human blood].
作者信息
Laferte J, Marrero M, Alvarez M, Jomarron L, Garcia S, Vazquez S, Morier L, Ulacia M, Melchor A
机构信息
Departmento de Virologia, Instituto de Medicina Tropical, Pedro Kouri Siboney, Ciudad Habana, Cuba.
出版信息
Rev Inst Med Trop Sao Paulo. 1992 Jan-Feb;34(1):43-7.
We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (1:40) and anti-human conjugate working dilution (1:1500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indirect immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.
我们已经标准化了一种间接超微量酶联免疫吸附测定法，用于使用人血清样本检测抗人巨细胞病毒（CMV）抗体（UMELISA CMV）。通过棋盘滴定法确定了包被抗原的最佳浓度（30μg/ml）、血清稀释度（1:40）和抗人缀合物工作稀释度（1:1500）。将UMELISA CMV与用于检测CMV抗体的乳胶凝集试验（杜邦公司）和间接免疫荧光法进行了比较。结果表明，与所比较的两种方法相比，所提出的测定法具有高度的一致性、敏感性和特异性，并支持其用于献血者筛查或通过配对血清样本进行该感染的血清学诊断。
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