Sen B H, Kazemi R B, Spångberg L S
Department of Restorative Dentistry & Endodontology, School of Dentistry, Ege University, Izmir, Turkey.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998 Sep;86(3):341-6. doi: 10.1016/s1079-2104(98)90183-4.
The aim of this study was to investigate the effect of titanium tetrafluoride solution on L929 fibroblasts by scanning electron microscopy. Titanium tetrafluoride was then compared with sodium fluoride and acidulated phosphate fluoride.
Cells were treated with fluoride solutions for 1 minute either directly, through a filter membrane with a pore size of 0.4-micron, or indirectly, through dentin disks; they were then investigated at an electron microscopic level.
Fluoride application on smeared dentin disks showed fewer cytotoxic effects on fibroblasts than application on nonsmeared dentin disks. Acidulated phosphate fluoride and titanium tetrafluoride appeared to be more cytotoxic than sodium fluoride. Because all fluoride solutions used in this study contained the same fluoride concentration, pH was considered to be the main factor causing the higher toxicity.
Because these solutions demonstrated toxicity in vitro, they must be further evaluated under in vivo conditions to ascertain their clinical safety.
本研究旨在通过扫描电子显微镜研究四氟化钛溶液对L929成纤维细胞的影响。然后将四氟化钛与氟化钠和酸化磷酸氟化物进行比较。
细胞通过直接、经孔径为0.4微米的滤膜或间接、经牙本质片用氟溶液处理1分钟；然后在电子显微镜水平进行研究。
与在未涂抹牙本质片上应用相比，在涂抹牙本质片上应用氟化物对成纤维细胞的细胞毒性作用更小。酸化磷酸氟化物和四氟化钛似乎比氟化钠具有更高的细胞毒性。由于本研究中使用的所有氟溶液含有相同的氟浓度，因此pH值被认为是导致更高毒性的主要因素。
由于这些溶液在体外表现出毒性，因此必须在体内条件下进一步评估以确定其临床安全性。
