Breath hydrogen measurement in ponies: a preliminary study.

The aim of this study was to establish the normal patterns of breath hydrogen excretion in adult ponies following either voluntary consumption or administration per stomach tube of a test meal/carbohydrate substrate. After an overnight fast, the ponies (n = 7) received either no test meal (ie fasted), glucose, xylose, lactose, lactulose, wheat flour or oats. Exhaled breath samples were collected in duplicate at 0 minutes and at 30-minute intervals thereafter for eight hours. Analysis of duplicate breath hydrogen measurements (n = 714) indicated that the breath hydrogen collection/storage method was reliable. Fasting resulted in negligible levels of breath hydrogen excretion. Increases in breath hydrogen concentration greater than 10 ppm, sustained for at least 30 minutes, were regarded as being biologically significantly different than fasting data and were observed for all ponies following the ingestion of oats or the administration of wheat flour, for three ponies following the administration of glucose and xylose and for two ponies following the administration of lactulose and lactose. The pattern of breath hydrogen excretion was subject to variation between animals following the ingestion of identical test meals. This study provides evidence of incomplete glucose absorption in healthy ponies and indicates that ingestion of non-absorbable carbohydrates do not always result in hydrogen excretion.