Broom A K, Hall R A, Johansen C A, Oliveira N, Howard M A, Lindsay M D, Kay B H, Mackenzie J S
Department of Microbiology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Australia.
Pathology. 1998 Aug;30(3):286-8. doi: 10.1080/00313029800169456.
An ELISA using a panel of specific monoclonal antibodies was developed to identify all alpha and flaviviruses isolated from mosquitoes caught throughout Australia. This technique is sensitive and rapid and is more specific than the traditional methods used to identify flaviviruses. The ability to identify unknown virus isolates from field-caught mosquitoes quickly and accurately improves the efficiency of arbovirus surveillance programs and allows health authorities to give an early warning of an increased health risk from a mosquito-borne virus in a particular region.
开发了一种使用一组特异性单克隆抗体的酶联免疫吸附测定法（ELISA），以鉴定从澳大利亚各地捕获的蚊子中分离出的所有甲病毒和黄病毒。该技术灵敏且快速，比用于鉴定黄病毒的传统方法更具特异性。能够快速、准确地从野外捕获的蚊子中鉴定出未知病毒分离株，提高了虫媒病毒监测计划的效率，并使卫生当局能够就特定地区蚊媒病毒导致的健康风险增加发出早期预警。
