Roehrig J T
J Gen Virol. 1982 Nov;63 (Pt 1):237-40. doi: 10.1099/0022-1317-63-1-237.
The applicability of the standard enzyme-linked immunosorbent assay (ELISA) for the identification of togavirus infections was investigated. Optimal concentration of gradient-purified antigen was 2.5 micrograms/well for alphaviruses or flaviviruses when coating polystyrene microtitre plates. A procedure for producing antigen in suckling mouse brain was developed. Results obtained with ELISA could be correlated with standard serology, but in general the ELISA was more sensitive. The ELISA was specific in differentiating alphavirus antigens. Flavivirus cross-reactivity was magnified by ELISA. ELISA should be useful as a rapid screening assay for non-related antigens, avoiding the extensive techniques currently used in standard serodiagnosis.
研究了标准酶联免疫吸附测定法（ELISA）在鉴定披膜病毒感染中的适用性。用梯度纯化抗原包被聚苯乙烯微量滴定板时，对于甲病毒或黄病毒，最佳浓度为2.5微克/孔。开发了一种在乳鼠脑中生产抗原的方法。ELISA获得的结果与标准血清学结果相关，但总体而言ELISA更敏感。ELISA在区分甲病毒抗原方面具有特异性。ELISA放大了黄病毒的交叉反应性。ELISA作为一种用于检测非相关抗原的快速筛选测定法应是有用的，可避免目前标准血清诊断中使用的繁琐技术。
