Otto M, Peschel A, Götz F
Universität Tübingen, Germany.
FEMS Microbiol Lett. 1998 Sep 15;166(2):203-11. doi: 10.1111/j.1574-6968.1998.tb13891.x.
Self-protection of the epidermin-producing strain Staphylococcus epidermidis Tü3298 against the pore-forming lantibiotic epidermin is mediated by an ABC transporter composed of the EpiF, EpiE, and EpiG proteins. We developed a sensitive assay based on HPLC analysis to investigate the capacity of the EpiFEG transporter to release epidermin and analogues from the cell surface to the external fluid. Our results indicate that the EpiFEG transporter works by expelling the lantibiotic from the cytoplasmic membrane into the surrounding medium. Analysis of transporter efficacy using nisin and gallidermin derivatives as substrates revealed a high substrate specificity. Furthermore, we showed that the activity of the gallidermin derivative L6G is enhanced by the presence of EpiE.
产生表皮菌素的表皮葡萄球菌菌株Tü3298对成孔羊毛硫抗生素表皮菌素的自我保护作用由EpiF、EpiE和EpiG蛋白组成的ABC转运蛋白介导。我们开发了一种基于高效液相色谱分析的灵敏检测方法，以研究EpiFEG转运蛋白将表皮菌素及其类似物从细胞表面释放到外部液体中的能力。我们的结果表明，EpiFEG转运蛋白通过将羊毛硫抗生素从细胞质膜排出到周围介质中发挥作用。以乳链菌肽和加里德明衍生物为底物分析转运蛋白的功效，结果显示出高底物特异性。此外，我们还表明，EpiE的存在增强了加里德明衍生物L6G的活性。
