Hasegawa N, Arai H, Igarashi Y
Department of Biotechnology, University of Tokyo, Japan.
FEMS Microbiol Lett. 1998 Sep 15;166(2):213-7. doi: 10.1111/j.1574-6968.1998.tb13892.x.
Expression of the enzymes for anaerobic nitrate respiration of Pseudomonas aeruginosa requires two CRP/FNR-related transcriptional regulators, ANR and DNR. Activity of the consensus CRP- or FNR-dependent promoter in the anr and dnr deficient mutants was investigated. The CRP-dependent promoter was active in the mutant strains. Both regulators could activate the promoter with a consensus FNR-binding motif. DNR-dependent activation was nitrite-dependent, whereas activation by ANR was not, suggesting that only DNR is involved in sensing nitrogen oxides.
铜绿假单胞菌厌氧硝酸盐呼吸酶的表达需要两种与CRP/FNR相关的转录调节因子，即ANR和DNR。研究了anr和dnr缺陷突变体中共识CRP或FNR依赖性启动子的活性。CRP依赖性启动子在突变菌株中具有活性。两种调节因子都可以激活具有共有FNR结合基序的启动子。DNR依赖性激活是亚硝酸盐依赖性的，而ANR的激活则不是，这表明只有DNR参与了氮氧化物的感知。
