Arai H, Igarashi Y, Kodama T
Department of Biotechnology, University of Tokyo, Japan.
FEBS Lett. 1995 Aug 28;371(1):73-6. doi: 10.1016/0014-5793(95)00885-d.
A gene, designated dnr, was identified in the vicinity of the structural genes for nitrite reductase (nirS) and nitric oxide reductase (norCB), and the gene for activation of the reductases (nirQ) from Pseudomonas aeruginosa. It encodes a protein of 227 amino acids homologous with the CRP/FNR-family transcriptional regulators. Promoter activities for nirS, nirQ and norCB were considerably reduced in the dnr mutant as well as in the mutant of anr, the other fnr-like regulatory gene from P. aeruginosa. This is the first finding that two CRP/FNR-related regulators are involved in denitrification in one strain.
在铜绿假单胞菌中，一个名为dnr的基因在亚硝酸还原酶（nirS）、一氧化氮还原酶（norCB）以及还原酶激活基因（nirQ）的结构基因附近被鉴定出来。它编码一个由227个氨基酸组成的蛋白质，该蛋白质与CRP/FNR家族转录调节因子同源。在dnr突变体以及来自铜绿假单胞菌的另一个fnr样调节基因anr的突变体中，nirS、nirQ和norCB的启动子活性都显著降低。这是首次发现两种与CRP/FNR相关的调节因子参与同一菌株的反硝化作用。
