Takamatsu H, Hiraoka T, Kodama T, Koide H, Kozuka S, Tochikubo K, Watabe K
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
FEMS Microbiol Lett. 1998 Sep 15;166(2):361-7. doi: 10.1111/j.1574-6968.1998.tb13913.x.
A DNA fragment (2.7 kbp) containing three deduced open reading frames, orf1, orf2 and orf3 (partial sequence), was isolated from the genomic library of Bacillus subtilis using an antiserum raised against spore integument, and was sequenced. orf2 was 519 nucleotides long and encoded a protein of 172 amino acids with a predicted molecular size of 19,552, corresponding to the protein which reacted with the antiserum. Immunoelectron microscopic observation indicated that YrbB, the product of orf2, was located within the spore integument, mainly in the cortex layer with a part in the inner region of the coat layer.
使用针对芽孢外皮产生的抗血清，从枯草芽孢杆菌的基因组文库中分离出一个包含三个推导的开放阅读框orf1、orf2和orf3（部分序列）的DNA片段（2.7 kbp），并对其进行了测序。orf2长519个核苷酸，编码一个由172个氨基酸组成的蛋白质，预测分子大小为19,552，与与抗血清反应的蛋白质相对应。免疫电子显微镜观察表明，orf2的产物YrbB位于芽孢外皮内，主要在皮层，部分位于外壳层的内部区域。
