叶绿素a加氧酶（CAO）参与叶绿素a向叶绿素b的转化过程。
Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a.
作者信息
Tanaka A, Ito H, Tanaka R, Tanaka N K, Yoshida K, Okada K
机构信息
The Institute of Low Temperature Science, Hokkaido University, Sapporo 0600819, Japan.
出版信息
Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12719-23. doi: 10.1073/pnas.95.21.12719.
Abstract
Chlorophyll b is an ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. Its biosynthesis plays a key role in the adaptation to various light environments. We isolated six chlorophyll b-less mutants by insertional mutagenesis by using the nitrate reductase or argininosuccinate lyase genes as tags and examined the rearrangement of mutant genomes. We found that an overlapping region of a nuclear genome was deleted in all mutants and that this encodes a protein whose sequence is similar to those of methyl monooxygenases. This coding sequence also contains putative binding domains for a [2Fe-2S] Rieske center and for a mononuclear iron. The results demonstrate that a chlorophyll a oxygenase is involved in chlorophyll b formation. The reaction mechanism of chlorophyll b formation is discussed.
摘要
叶绿素b是陆地植物、绿藻和原绿藻中普遍存在的辅助色素。其生物合成在适应各种光照环境中起着关键作用。我们通过使用硝酸还原酶或精氨琥珀酸裂解酶基因作为标签进行插入诱变，分离出了六个无叶绿素b的突变体，并检测了突变体基因组的重排。我们发现所有突变体中一个核基因组的重叠区域被删除，该区域编码一种蛋白质，其序列与甲基单加氧酶的序列相似。该编码序列还包含一个[2Fe-2S] Rieske中心和一个单核铁的假定结合域。结果表明，叶绿素a加氧酶参与叶绿素b的形成。本文还讨论了叶绿素b形成的反应机制。
相似文献
1
Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a.叶绿素a加氧酶（CAO）参与叶绿素a向叶绿素b的转化过程。
Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12719-23. doi: 10.1073/pnas.95.21.12719.
2
Synthesis of chlorophyll b: localization of chlorophyllide a oxygenase and discovery of a stable radical in the catalytic subunit.叶绿素b的合成：叶绿素酸酯a加氧酶的定位及催化亚基中稳定自由基的发现
BMC Plant Biol. 2004 Apr 15;4:5. doi: 10.1186/1471-2229-4-5.
3
Domain structures of chlorophyllide a oxygenase of green plants and Prochlorothrix hollandica in relation to catalytic functions.绿色植物和荷兰原绿藻叶绿素酸a加氧酶的结构域与催化功能的关系
Planta. 2004 Apr;218(6):1019-25. doi: 10.1007/s00425-003-1181-6. Epub 2004 Jan 10.
4
Differential regulation of chlorophyll a oxygenase genes in rice.水稻中叶绿素a加氧酶基因的差异调控
Plant Mol Biol. 2005 Apr;57(6):805-18. doi: 10.1007/s11103-005-2066-9.
5
The AtCAO gene, encoding chlorophyll a oxygenase, is required for chlorophyll b synthesis in Arabidopsis thaliana.编码叶绿素a加氧酶的AtCAO基因是拟南芥叶绿素b合成所必需的。
Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10507-11. doi: 10.1073/pnas.96.18.10507.
6
Evolutionary changes in chlorophyllide a oxygenase (CAO) structure contribute to the acquisition of a new light-harvesting complex in micromonas.叶绿素 a 加氧酶（CAO）结构的进化变化有助于在微小单胞菌中获得一个新的光捕获复合物。
J Biol Chem. 2013 Jul 5;288(27):19330-41. doi: 10.1074/jbc.M113.462663. Epub 2013 May 15.
7
Functional analysis of N-terminal domains of Arabidopsis chlorophyllide a oxygenase.拟南芥叶绿素酸a加氧酶N端结构域的功能分析
Plant Physiol Biochem. 2007 Oct-Nov;45(10-11):740-9. doi: 10.1016/j.plaphy.2007.07.016. Epub 2007 Jul 29.
8
Structural Characterization of the Chlorophyllide a Oxygenase (CAO) Enzyme Through an In Silico Approach.通过计算机模拟方法对叶绿素酸酯a加氧酶（CAO）进行结构表征。
J Mol Evol. 2023 Apr;91(2):225-235. doi: 10.1007/s00239-023-10100-9. Epub 2023 Mar 3.
9
Clp protease controls chlorophyll b synthesis by regulating the level of chlorophyllide a oxygenase.Clp蛋白酶通过调节叶绿素酸a加氧酶的水平来控制叶绿素b的合成。
Plant J. 2007 Mar;49(5):800-9. doi: 10.1111/j.1365-313X.2006.02996.x. Epub 2007 Feb 9.
10
Determination of a chloroplast degron in the regulatory domain of chlorophyllide a oxygenase.确定叶绿素 a 加氧酶调节结构域中的叶绿体降解序列。
J Biol Chem. 2009 Dec 25;284(52):36689-36699. doi: 10.1074/jbc.M109.008144. Epub 2009 Oct 20.
引用本文的文献
1
Applying an Anti-Kasha Model Resolves Differences Between Photosynthetic and Artificial Pigments.应用反卡莎模型解决光合色素与人工色素之间的差异。
J Phys Chem B. 2025 Aug 7;129(31):7884-7895. doi: 10.1021/acs.jpcb.5c02465. Epub 2025 Jul 23.
2
Regulatory and retrograde signaling networks in the chlorophyll biosynthetic pathway.叶绿素生物合成途径中的调控与逆向信号网络。
J Integr Plant Biol. 2025 Apr;67(4):887-911. doi: 10.1111/jipb.13837. Epub 2025 Jan 24.
3
Light quality affects chlorophyll biosynthesis and photosynthetic performance in Antarctic Chlamydomonas.光质影响南极衣藻的叶绿素生物合成和光合性能。
Photosynth Res. 2025 Jan 20;163(1):9. doi: 10.1007/s11120-024-01127-0.
4
Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium.当将衣藻细胞转移到缺铁培养基中 48 小时内，细胞会经历明显的铁营养阶段转变。
Photosynth Res. 2024 Sep;161(3):213-232. doi: 10.1007/s11120-024-01103-8. Epub 2024 Jul 17.
5
Knockout of the Chlorophyll a Oxygenase Gene Reduces Chilling Tolerance in Rice Seedlings.叶绿素 a 加氧酶基因敲除降低水稻幼苗的抗冷性。
Genes (Basel). 2024 Jun 2;15(6):721. doi: 10.3390/genes15060721.
6
Metal-tolerant isolates can potentially mediate nickel stress tolerance in Arabidopsis by upregulating antioxidative enzyme activities.耐金属的分离株可以通过上调抗氧化酶活性来潜在介导拟南芥对镍胁迫的耐受。
Plant Signal Behav. 2024 Dec 31;19(1):2318513. doi: 10.1080/15592324.2024.2318513. Epub 2024 Mar 25.
7
Reduced expression of chlorophyllide oxygenase (CAO) decreases the metabolic flux for chlorophyll synthesis and downregulates photosynthesis in tobacco plants.叶绿素酸酯加氧酶（CAO）表达量降低会减少烟草植株中叶绿素合成的代谢通量，并下调光合作用。
Physiol Mol Biol Plants. 2024 Jan;30(1):1-16. doi: 10.1007/s12298-023-01395-5. Epub 2023 Dec 3.
8
A Genome-Wide Association Study Reveals the Genetic Mechanisms of Nutrient Accumulation in Spinach.一项全基因组关联研究揭示了菠菜中营养物质积累的遗传机制。
Genes (Basel). 2024 Jan 28;15(2):172. doi: 10.3390/genes15020172.
9
Carbon monoxide is involved in melatonin-enhanced drought resistance in tomato seedlings by enhancing chlorophyll synthesis pathway.一氧化碳通过增强叶绿素合成途径参与褪黑素增强番茄幼苗的抗旱性。
BMC Plant Biol. 2024 Feb 9;24(1):97. doi: 10.1186/s12870-024-04793-3.
10
Modeling the Characteristic Residues of Chlorophyll Synthase (ChlF) from to Determine Its Reaction Mechanism.通过建模分析叶绿素合酶（ChlF）的特征残基以确定其反应机制。
Microorganisms. 2023 Sep 13;11(9):2305. doi: 10.3390/microorganisms11092305.
本文引用的文献
1
Chlorophyll Biosynthesis.叶绿素生物合成
Plant Cell. 1995 Jul;7(7):1039-1057. doi: 10.1105/tpc.7.7.1039.
2
Correlated Changes in the Activity, Amount of Protein, and Abundance of Transcript of NADPH:Protochlorophyllide Oxidoreductase and Chlorophyll Accumulation during Greening of Cucumber Cotyledons.黄瓜子叶绿化过程中NADPH：原叶绿素酸酯氧化还原酶的活性、蛋白质含量、转录本丰度与叶绿素积累的相关变化
Plant Physiol. 1995 Sep;109(1):231-238. doi: 10.1104/pp.109.1.231.
3
Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2.睾丸酮丛毛单胞菌T-2中对甲苯磺酸盐操纵子tsaMBCD和tsaR的特性分析
J Bacteriol. 1997 Feb;179(3):919-27. doi: 10.1128/jb.179.3.919-927.1997.
4
Independent evolution of the prochlorophyte and green plant chlorophyll a/b light-harvesting proteins.原绿藻和绿色植物叶绿素a/b捕光蛋白的独立进化。
Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15244-8. doi: 10.1073/pnas.93.26.15244.
5
Coexistence of phycoerythrin and a chlorophyll a/b antenna in a marine prokaryote.海洋原核生物中藻红蛋白与叶绿素a/b天线的共存。
Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):11126-30. doi: 10.1073/pnas.93.20.11126.
6
The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.细菌博来霉素抗性基因ble作为衣藻中的显性选择标记。
Mol Gen Genet. 1996 Apr 24;251(1):23-30. doi: 10.1007/BF02174340.
7
Conversion of chlorophyll b to chlorophyll a via 7-hydroxymethyl chlorophyll.
J Biol Chem. 1996 Jan 19;271(3):1475-9. doi: 10.1074/jbc.271.3.1475.
8
The derivation of the formyl-group oxygen of chlorophyll b in higher plants from molecular oxygen. Achievement of high enrichment of the 7-formyl-group oxygen from 18O2 in greening maize leaves.高等植物中叶绿素b的甲酰基氧源自分子氧。在绿化玉米叶片中实现了用18O2对7-甲酰基氧的高度富集。
Eur J Biochem. 1994 Jan 15;219(1-2):671-9. doi: 10.1111/j.1432-1033.1994.tb19983.x.
9
Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis.通过DNA插入诱变克隆莱茵衣藻的鞭毛基因。
Genetics. 1993 Oct;135(2):375-84. doi: 10.1093/genetics/135.2.375.
10
Directed mutational analysis of bacteriochlorophyll a biosynthesis in Rhodobacter capsulatus.荚膜红细菌中细菌叶绿素a生物合成的定向突变分析。
J Mol Biol. 1994 Apr 15;237(5):622-40. doi: 10.1006/jmbi.1994.1260.
Zotero 插件