Ariel I, Hochberg A, Shochina M
Hadassah University Hospital and the Hebrew University-Hadassah Medical School, Jerusalem, Israel.
J Clin Pathol. 1998 Jun;51(6):427-31. doi: 10.1136/jcp.51.6.427.
To study the localisation of the endothelial nitric oxide synthase (eNOS) in the normal placenta, with special emphasis on the implantation site in the first trimester of pregnancy, and in the different subtypes of trophoblastic cells in gestational trophoblastic disease.
The immunoperoxidase technique with an antibody directed against eNOS was applied to paraffin sections from first and second trimester placentas, placenta accreta, partial and complete hydatidiform moles, and choriocarcinoma. Immunoperoxidase staining for human placental lactogen (hPL) was performed on parallel sections.
Prominent immunoreactivity for eNOS was found to be present in the intermediate trophoblastic cells of the cell columns of the anchoring villi and in trophoblastic cells at the implantation site. Staining was also present in the syncytiotrophoblast, most conspicuous at the apical cell border. In trophoblastic disease, proliferating large mononuclear cells, which were strongly positive for hPL, were found to be immunoreactive for eNOS.
eNOS immunoreactivity is strongly positive in the extravillous trophoblastic cells and to a lesser extent in the syncytiotrophoblast. In the former it may play a role in implantation and vascular invasion. Cells with differentiation to intermediate trophoblast in complete hydatidiform mole and choriocarcinoma also show high levels of eNOS, which may be associated with the haematogenous mode of spread of trophoblastic disease.
研究内皮型一氧化氮合酶（eNOS）在正常胎盘中的定位，特别关注妊娠早期的着床部位以及妊娠滋养细胞疾病中不同亚型滋养层细胞的情况。
将针对eNOS的抗体免疫过氧化物酶技术应用于孕早期和孕中期胎盘、胎盘植入、部分和完全性葡萄胎以及绒毛膜癌的石蜡切片。在平行切片上进行人胎盘催乳素（hPL）的免疫过氧化物酶染色。
发现eNOS在固定绒毛细胞柱的中间型滋养层细胞和着床部位的滋养层细胞中呈现显著的免疫反应性。合体滋养层细胞中也有染色，在顶端细胞边界处最为明显。在滋养细胞疾病中，发现对hPL呈强阳性的增殖性大单核细胞对eNOS也有免疫反应性。
eNOS免疫反应性在绒毛外滋养层细胞中呈强阳性，在合体滋养层细胞中程度较轻。在前者中，它可能在着床和血管侵入中起作用。完全性葡萄胎和绒毛膜癌中分化为中间型滋养层的细胞也显示出高水平的eNOS，这可能与滋养细胞疾病的血行播散方式有关。
