Klucis E, Jackson L, Parsons P G
Int J Cancer. 1976 Oct 15;18(4):413-20. doi: 10.1002/ijc.2910180404.
Velocity sedimentation of uridine-labelled cultures was found to be more reliable than isopycnic sedimentation in detecting oncornavirus production in lymphoid cells. Of 13 cell lines (including six derivea from Burkitt's lymphomas and two from leukaemic leukocytes) only one, the leukaemia-derived, Epstein-Barr virus-producing line QIMR-WIL, showed any activity. The nature of the QIMR-WIL particles was further defined by isolation of uridine-labelled 70S RNA and by the simultaneous assay for reverse transcriptase and 70S RNA, but production of such particles was detected in only three of 10 assays. Pretreatment of cells with 5'-iododeoxyuridine or culture in arginine-free medium did not induce particle production. Syncytia assays using XC cells were negative. Of 13 primary cultures (nine samples of leukaemic leukocytes and four of cord leukocytes) treated with mitogens and subjected to inducing conditions, one (leukocytes from a patient with acute myelogenous leukaemia) showed evidence in successive assays of oncornavirus synthesis. The low and transient yield of oncornavirus-like particles obtained in this work parallels that reported in previous studies of fresh lymphoid cells and primary cultures.
在检测淋巴细胞中的肿瘤病毒产生时，发现尿苷标记培养物的速度沉降比等密度沉降更可靠。在13个细胞系（包括6个源自伯基特淋巴瘤的细胞系和2个源自白血病白细胞的细胞系）中，只有一个源自白血病且产生爱泼斯坦-巴尔病毒的细胞系QIMR-WIL显示出任何活性。通过分离尿苷标记的70S RNA以及同时检测逆转录酶和70S RNA，进一步确定了QIMR-WIL颗粒的性质，但在10次检测中只有3次检测到了此类颗粒的产生。用5'-碘脱氧尿苷预处理细胞或在无精氨酸培养基中培养均未诱导颗粒产生。使用XC细胞的合胞体检测为阴性。在用有丝分裂原处理并置于诱导条件下的13个原代培养物（9个白血病白细胞样本和4个脐血白细胞样本）中，有一个（一名急性髓性白血病患者的白细胞）在连续检测中显示出肿瘤病毒合成的证据。在这项研究中获得的类似肿瘤病毒颗粒的低产量和短暂产量与先前对新鲜淋巴细胞和原代培养物的研究报道相似。
