Parsons P G, Klucis E, Goss P D, Pope J H, Little J H, Davis N C
Int J Cancer. 1976 Dec 15;18(6):757-63. doi: 10.1002/ijc.2910180606.
Tests for the presence of oncornavirus-like particles in human biopsies were made by the Spiegelman simultaneous assay for 70S RNA and RNA-dependent DNA polymerase and by detection of 600-900S particles, incorporating 3H-uridine, produced by cultured biopsy cells. Thirty-one malignant melanoma biopsies from 29 patients were studied. Using the simultaneous assay, evidence of virus-like particles was found in 15/26 (58%) of melanoma biopsies, 0/3 naevi pools, 1/4 samples of skin adjacent to melanoma, 0/3 samples of normal adult skin and 0/3 prepuces. The velocity sedimentation technique was shown to be a useful screening test for oncornaviruses in studies of two virus-producing mouse cell lines (TKL-5 and WEHI-22), and was positive with 7/9 melanoma biopsies. Overall, these results are compatible with the earlier findings of similar virus-like particles in malignant melanoma cell lines, but the exact nature of the particles remains to be defined.
通过Spiegelman同时检测70S RNA和RNA依赖性DNA聚合酶以及检测培养的活检细胞产生的掺入3H-尿苷的600 - 900S颗粒，对人类活检组织中是否存在类肿瘤病毒颗粒进行检测。研究了来自29名患者的31份恶性黑色素瘤活检组织。使用同时检测法，在26份黑色素瘤活检组织中的15份（58%）、3份痣样本中的0份、黑色素瘤旁皮肤样本中的1份、3份正常成人皮肤样本中的0份以及3份包皮样本中的0份中发现了病毒样颗粒的证据。在对两种产生病毒的小鼠细胞系（TKL - 5和WEHI - 22）的研究中，速度沉降技术被证明是一种用于筛查肿瘤病毒的有用检测方法，在9份黑色素瘤活检组织中的7份检测结果呈阳性。总体而言，这些结果与早期在恶性黑色素瘤细胞系中发现类似病毒样颗粒的结果一致，但这些颗粒的确切性质仍有待确定。
