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定量荧光细胞术标准化的术语和命名法。

Terminology and nomenclature for standardization in quantitative fluorescence cytometry.

作者信息

Henderson L O, Marti G E, Gaigalas A, Hannon W H, Vogt R F

机构信息

National Diabetes Reference Laboratory, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

出版信息

Cytometry. 1998 Oct 1;33(2):97-105. doi: 10.1002/(sici)1097-0320(19981001)33:2<97::aid-cyto3>3.0.co;2-h.

DOI:10.1002/(sici)1097-0320(19981001)33:2<97::aid-cyto3>3.0.co;2-h
PMID:9773869
Abstract

Terminology in any field is a complex mix of established conventions, accepted usages, disputed terms, and occasional misnomers. The terminology that has evolved for quantitative fluorescence cytometry (QFCM) is especially multifarious, in part because QFCM encompasses a range from subjective visual assessments to objective photon counts. Thus, while descriptive terms such as "dim" and "bright" are still quite useful, quantitative terms such as "binding capacity" should be used with collective understanding of their exact meanings. This article reviews current usage and proposes definitions that, with refinement from suppliers and users of QFCM technology, can provide the required clarity.

摘要

任何领域的术语都是既定惯例、公认用法、有争议的术语以及偶尔出现的误称的复杂混合体。为定量荧光细胞术(QFCM)发展而来的术语尤其繁杂,部分原因是QFCM涵盖了从主观视觉评估到客观光子计数的范围。因此,虽然诸如“暗”和“亮”等描述性术语仍然非常有用,但诸如“结合能力”等定量术语应在对其确切含义有共同理解的基础上使用。本文回顾了当前的用法,并提出了一些定义,经过QFCM技术供应商和用户的完善,可以提供所需的清晰度。

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