Dingman J R, Rayner M G, Mishra S, Zhang Y, Ehrlich M D, Post J C, Ehrlich G D
Center for Genomic Sciences, Allegheny University of the Health Sciences, Pittsburgh, Pennsylvania 15212, USA.
J Clin Microbiol. 1998 Nov;36(11):3417-9. doi: 10.1128/JCM.36.11.3417-3419.1998.
The presence of endotoxin (detected by the Limulus amebocyte lysate assay) was compared to the presence of viable Haemophilus influenzae and Moraxella catarrhalis (detected by PCR) in 106 middle-ear effusions from pediatric patients with chronic otitis media. Endotoxin was found in 81 of the 106 specimens. Of these 81 specimens, 66 (81.5%) also tested positive for one or both of the gram-negative bacteria H. influenzae and M. catarrhalis. The data suggest that viable gram-negative bacteria, detectable by PCR but often undetectable by culture, may be the source of endotoxin in middle-ear effusions.
通过鲎试剂检测法检测内毒素的存在情况,并与通过聚合酶链反应(PCR)检测的106例慢性中耳炎小儿患者中耳积液中流感嗜血杆菌和卡他莫拉菌的活菌存在情况进行比较。106份标本中有81份检测到内毒素。在这81份标本中,66份(81.5%)对革兰氏阴性菌流感嗜血杆菌和卡他莫拉菌中的一种或两种检测呈阳性。数据表明,通过PCR可检测到但通常培养无法检测到的革兰氏阴性活菌可能是中耳积液中内毒素的来源。