儿童分泌性中耳炎中耳生物膜形成及细菌学研究：一项前瞻性横断面研究
The Formation of Biofilm and Bacteriology in Otitis Media with Effusion in Children: A Prospective Cross-Sectional Study.
作者信息
Niedzielski Artur, Chmielik Lechosław Paweł, Stankiewicz Tomasz
机构信息
Clinic of Pediatric Otolaryngology, Center of Postgraduate Medical Education (CMKP), 01-813 Warsaw, Poland.
Independent Otoneurological Laboratory, Medical University of Lublin, 20-093 Lublin, Poland.
出版信息
Int J Environ Res Public Health. 2021 Mar 30;18(7):3555. doi: 10.3390/ijerph18073555.
BACKGROUND
Otitis media with effusion (OME) can cause serious complications such as hearing impairment or development delays. The aim of the study was to assess the microbiological profile of organisms responsible for OME and to determine if a biofilm formation can be observed.
METHODS
Ninety-nine samples from 76 patients aged from 6 months to 12 years were collected for microbiological and molecular studies.
RESULTS
In microbiological studies, pathogenic bacteria (38.89%), (33.33%), and (27.78%), as well as opportunistic bacteria spp. (74.14%), (20.69%), (3.45%), and spp. (1.72%) were found. The average degree of hearing loss in the group of children with positive bacterial culture was 35.9 dB, while in the group with negative bacterial culture it was 25.9 dB ( = 0.0008). The type of cultured bacteria had a significant impact on the degree of hearing impairment in children ( = 0.0192). In total, 37.5% of spp. strains were able to form biofilm.
CONCLUSIONS
spp. in OME may form biofilms, which can explain the chronic character of the disease. Pathogenic and opportunistic bacteria may be involved in the etiopathogenesis of OME. The degree of hearing loss was significantly higher in patients from which the positive bacterial cultures were obtained.
背景
中耳积液（OME）可导致严重并发症，如听力障碍或发育迟缓。本研究的目的是评估导致OME的微生物谱，并确定是否能观察到生物膜形成。
方法
收集了76例年龄在6个月至12岁之间的患者的99份样本，用于微生物学和分子研究。
结果
在微生物学研究中，发现了病原菌（38.89%）、（33.33%）和（27.78%），以及机会致病菌属（74.14%）、（20.69%）、（3.45%）和属（1.72%）。细菌培养阳性的儿童组平均听力损失程度为35.9dB，而细菌培养阴性的儿童组平均听力损失程度为25.9dB（=0.0008）。培养出的细菌类型对儿童听力损伤程度有显著影响（=0.0192）。总的来说，37.5%的菌株能够形成生物膜。
结论
OME中的菌可能形成生物膜，这可以解释该疾病的慢性特征。病原菌和机会致病菌可能参与了OME的发病机制。获得细菌培养阳性结果的患者听力损失程度明显更高。
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