Willett D N, Rezaee R P, Billy J M, Tighe M B, DeMaria T F
Department of Otolaryngology, Ohio State University College of Medicine, Columbus 43210, USA.
Ann Otol Rhinol Laryngol. 1998 Jan;107(1):28-33. doi: 10.1177/000348949810700106.
Sixty-five middle ear effusions and paired sera from 41 children with chronic otitis media with effusion were assayed for endotoxin and for tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in order to establish whether a correlation exists between the concentrations of endotoxin and of these cytokines. Endotoxin concentration was determined by means of a chromogenic limulus amebocyte lysate assay, and the cytokine concentration by means of a quantitative enzyme-linked immunosorbent assay. Forty percent of the effusions had detectable levels of endotoxin, with a mean concentration of 2.9 +/- 7.8 endotoxin units per milligram of total protein. The mean concentration of TNF-alpha was 1.24 +/- 3.1 pg/mg total protein, and that of IL-1 beta was 18.79 pg/mg total protein. A strong, statistically significant correlation exists between the concentrations of endotoxin and TNF-alpha (r = .89) and IL-1 beta (r = .72). The data indicate that endotoxin may contribute to the pathogenesis of chronic otitis media with effusion by stimulating the sustained production of TNF-alpha and IL-1 beta in the middle ear.
对65份中耳积液以及来自41名分泌性中耳炎患儿的配对血清进行内毒素、肿瘤坏死因子α（TNF-α）和白细胞介素-1β（IL-1β）检测，以确定内毒素浓度与这些细胞因子浓度之间是否存在相关性。内毒素浓度通过显色鲎试剂法测定，细胞因子浓度通过定量酶联免疫吸附测定法测定。40%的积液内毒素水平可检测到，总蛋白每毫克的平均浓度为2.9±7.8内毒素单位。TNF-α的平均浓度为1.24±3.1 pg/mg总蛋白，IL-1β的平均浓度为18.79 pg/mg总蛋白。内毒素浓度与TNF-α（r = 0.89）和IL-1β（r = 0.72）之间存在强的、具有统计学意义的相关性。数据表明，内毒素可能通过刺激中耳持续产生TNF-α和IL-1β，从而促进分泌性中耳炎的发病机制。
