Tachibana T, Matsuyama T, Mitsuyama M
Department of Bacteriology, Niigata University School of Medicine, Niigata 951, Japan; Department of Dermatology, Niigata University School of Medicine, Niigata 951, Japan.
Med Mycol. 1998 Feb;36(1):21-7.
Isolates of Sporothrix schenckii were examined for their infectivity in BALB/c mice. The mice were injected with yeast forms of S. schenckii isolates differing in clinical source (human cutaneous lesions and pulmonary lesions), and fungal growth was determined at intervals in the footpad and visceral organs. After subcutaneous injection of approximately 10 colony forming units (cfu) of S. schenckii into the footpad, locally restricted fungal infection developed gradually. At the peak of the infection (3-4 weeks post-inoculation), viable fungal counts reached 102-106 cfu/footpad. Dissemination to other tissues and visceral organs was not observed. After intravenous or intraperitoneal injection of 106 cfu of yeast forms, three of four isolates from cutaneous sporotrichosis were unable to establish infection and were eliminated from blood and visceral organs. The development of systemic infection was observed only with S. schenckii isolates obtained from the human lung lesion. Thus, inherent properties of each clinical isolate and routes of infection were shown to be critical for the establishment of systemic infection in spite of the remarkably strong infectivity of S. schenckii to the cutaneous tissue.
对申克孢子丝菌分离株在BALB/c小鼠中的感染性进行了检测。给小鼠注射临床来源不同（人类皮肤病变和肺部病变）的申克孢子丝菌分离株的酵母形式，并定期测定足垫和内脏器官中的真菌生长情况。将约10个菌落形成单位（cfu）的申克孢子丝菌皮下注射到足垫后，局部受限的真菌感染逐渐发展。在感染高峰期（接种后3 - 4周），活真菌计数达到10² - 10⁶ cfu/足垫。未观察到向其他组织和内脏器官的播散。静脉注射或腹腔注射10⁶ cfu酵母形式后，来自皮肤孢子丝菌病的四个分离株中有三个无法建立感染，并从血液和内脏器官中清除。仅从人类肺部病变获得的申克孢子丝菌分离株观察到全身感染的发展。因此，尽管申克孢子丝菌对皮肤组织具有极强的感染性，但每个临床分离株的固有特性和感染途径对于全身感染的建立至关重要。
