Nobre Márcia de Oliveira, Antunes Tatiana de Avila, de Faria Renata Osório, Cleff Marlete Brum, Fernandes Cristina Gevehr, Muschner Adriana Cunha, Meireles Mário Carlos Araújo, Ferreiro Laerte
Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Avemda Bento Gonçalves no. 9090-91540-000, Porto Alegre, RS-Brazil.
Mycopathologia. 2005 Aug;160(1):43-9. doi: 10.1007/s11046-005-6866-x.
Sporotrichosis is a chronic subcutaneous mycosis caused by Sporothrix schenckii. This work aimed to evaluate the virulence of two different isolates of S. schenckii from cutaneous (CUT) and systemic (SYS) forms of feline sporotrichosis. A standard inoculum with 2 x 10(3) yeast cells/ml was prepared from each of the isolates. The experimental infection was carried out with 0.1 ml of the inoculum from both isolates and then injected in the paw pads of Swiss albino mice of groups CUT and SYS. The clinical evolution of the disease and the diameter of the lesion at the inoculated sites were evaluated during nine weeks. Four necropsies were done to collect material from the lesions (p < 0.01). Group CUT demonstrated a more evident clinical evolution of the disease from week two to week five; large lesions in the paw pad on week four (p < 0.01); and a higher incidence of lesions in other parts of the body (p < 0.01) than group SYS (p < 0.01). S. schenckii was isolated from the inoculated site in groups SYS and CUT until days 30 and 45, respectively. Granulomas with yeast cells usually localized in the central area were observed in histopathology sections on days 15 and 30 post-inoculations. Those yeast cells decreased on day 45 being absent on day 62 when tissue repair initiated. The results showed that distinct clinical isolates of S. schenckii cause significant differences in the clinical evolution of sporotrichosis.
孢子丝菌病是由申克孢子丝菌引起的一种慢性皮下真菌病。这项研究旨在评估从猫孢子丝菌病的皮肤型（CUT）和系统型（SYS）分离出的两种不同申克孢子丝菌菌株的毒力。从每种菌株制备了浓度为2×10³个酵母细胞/毫升的标准接种物。用0.1毫升来自两种菌株的接种物进行实验性感染，然后注射到CUT组和SYS组的瑞士白化小鼠的爪垫中。在九周内评估疾病的临床进展以及接种部位病变的直径。进行了四次尸检以收集病变部位的材料（p<0.01）。CUT组在第2周和第5周疾病的临床进展更为明显；第4周爪垫出现大的病变（p<0.01）；与SYS组相比，身体其他部位病变的发生率更高（p<0.01）。在SYS组和CUT组中，分别直到第30天和第45天从接种部位分离出申克孢子丝菌。在接种后第15天和第30天的组织病理学切片中观察到通常位于中央区域的含有酵母细胞的肉芽肿。这些酵母细胞在第45天减少，在第62天组织修复开始时消失。结果表明，申克孢子丝菌的不同临床分离株在孢子丝菌病的临床进展中引起显著差异。
