O'Hare M J, Nice E C, Magee-Brown R, Bullman H
J Chromatogr. 1976 Sep 29;125(1):357-67. doi: 10.1016/s0021-9673(00)93831-7.
Reversed-phase high-pressure liquid chromatography with gradient elution on Zorbax-ODS columns has been used to separate, identify, and measure, spectrophotometrically, the steroids secreted by both human adrenal and testis cells in primary monolayer culture. Three related systems using exponential concave gradients have been developed with the specific objective of resolving the steroids produced by these two tissues. A methanol-water gradient has been used to separate most adrenal steroids, an acetonitrile-water gradient to separate testis steroids, and a dioxane-water gradient to separate polar steroids, including aldosterone. These three systems together permit the resolution of at least 43 naturally occurring steroids, plus four synthetic steroids with adrenocortical activity, with overall total elution times of 1 h or less for each system. Retention data for these steroids are given and the separation of steroids in the biological samples illustrated.
采用Zorbax-ODS柱上梯度洗脱的反相高效液相色谱法，对原代单层培养的人肾上腺和睾丸细胞分泌的甾体进行了分离、鉴定和分光光度测定。为了分离这两种组织产生的甾体，已开发出三种使用指数凹形梯度的相关系统。甲醇-水梯度用于分离大多数肾上腺甾体，乙腈-水梯度用于分离睾丸甾体，二氧六环-水梯度用于分离包括醛固酮在内的极性甾体。这三种系统合在一起可分离至少43种天然存在的甾体，外加四种具有肾上腺皮质活性的合成甾体，每个系统的总洗脱时间均为1小时或更短。给出了这些甾体的保留数据，并展示了生物样品中甾体的分离情况。
