16-脱氢甾体生物合成的研究。[4-¹⁴C]孕烯醇酮在体外经公猪肾上腺和睾丸组织的代谢。
Studies on the biosynthesis of 16-dehydro steroids. The metabolism of [4-14C]pregnenolone by boar adrenal and testis tissue in vitro.
作者信息
Gower D B, Ahmad N
出版信息
Biochem J. 1967 Aug;104(2):550-6. doi: 10.1042/bj1040550.
Abstract
- The metabolism of [4-(14)C]pregnenolone in vitro by boar adrenocortical and testis tissue has been studied. 2. Boar testis tissue formed three labelled Delta(16)-steroids, 5alpha-androst-16-en-3alpha-ol, 5alpha-androst-16-en-3beta-ol and androsta-4,16-dien-3-one. In adrenal tissue very much smaller yields of the same metabolites were obtained. 3. Both tissues produced labelled progesterone, androst-4-ene-3,17-dione and testosterone in varying quantities. The amount of progesterone was about 120 times greater in the adrenal tissue. In testis tissue dehydroepiandrosterone was found only in small quantity. 4. A pathway is suggested for the biosynthesis of Delta(16)-steroids from pregnenolone in boar testis tissue. The possibility that progesterone may be an intermediate is discussed.
摘要
- 已对猪肾上腺皮质和睾丸组织在体外对[4-(14)C]孕烯醇酮的代谢进行了研究。2. 猪睾丸组织形成了三种标记的Δ(16)-类固醇，即5α-雄甾-16-烯-3α-醇、5α-雄甾-16-烯-3β-醇和雄甾-4,16-二烯-3-酮。在肾上腺组织中，相同代谢产物的产量要少得多。3. 两种组织都产生了标记的孕酮、雄甾-4-烯-3,17-二酮和睾酮，但数量各不相同。肾上腺组织中孕酮的量约为睾丸组织的120倍。在睾丸组织中，仅发现少量脱氢表雄酮。4. 提出了一条从孕烯醇酮在猪睾丸组织中生物合成Δ(16)-类固醇的途径。讨论了孕酮可能作为中间体的可能性。
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