Petersen B A, Hanson R N, Giese R W, Karger B L
J Chromatogr. 1976 Nov 3;126:503-16. doi: 10.1016/s0021-9673(01)84097-8.
A methodology involving equilibrium dialysis and gas chromatography has been developed to measure concurrently the concentration of the dialyzable (i.e., free) fractions of thyroxine and 3,5,3'-triiodothyronine in serum. The electron capture response of the N,0-diheptafluorobutyryl methyl ester derivatives of these hormones allows their detection and quantitation in the picogram range (detection limit approx. 0.2pg). Purification of the dialysate is achieved using a cation-exchange column which is pretreated with diiodothyronine. Adsorption problems on the glassware are eliminated by silanization. The methodology also resolves 3,3',5'-triiodothyronine (reverse T3).
已开发出一种涉及平衡透析和气相色谱的方法，用于同时测量血清中甲状腺素和3,5,3'-三碘甲状腺原氨酸的可透析（即游离）部分的浓度。这些激素的N,0-二庚氟丁酰甲酯衍生物的电子捕获响应使其能够在皮克范围内进行检测和定量（检测限约为0.2皮克）。使用用二碘甲状腺原氨酸预处理的阳离子交换柱对透析液进行纯化。通过硅烷化消除玻璃器皿上的吸附问题。该方法还可分离出3,3',5'-三碘甲状腺原氨酸（反式T3）。
