Tesch J W, Rehg W R, Sievers R E
J Chromatogr. 1976 Nov 3;126:743-55. doi: 10.1016/s0021-9673(01)84117-0.
A generally applicable method for the analysis of nitrates and nitrites has been used for a wide variety of samples, including human saliva, blood, drinking water, and airborne particulates. Aqueous nitrate ion is first converted to nitrobenzene by reaction with benzene (or another aromatic reactant) in the presence of a catalyst. The nitrobenzene is then quantitated by electron capture gas chromatography (GC-ECD). Nitrite ion and gaseous oxides of nitrogen can be determined also if such samples are treated appropriately prior to CG-ECD analysis. Other reactants such as 1,3,5-trimethoxybenzene can also be used in the place of benzene. The high sensitivity of ECD allows the quantitation of as little as 0.1 ppm (w/w) nitrate in a single drop of saliva or blood.
一种普遍适用的硝酸盐和亚硝酸盐分析方法已被用于多种样品，包括人类唾液、血液、饮用水和空气中的颗粒物。在催化剂存在下，硝酸根离子首先通过与苯（或另一种芳香族反应物）反应转化为硝基苯。然后通过电子捕获气相色谱法（GC-ECD）对硝基苯进行定量。如果此类样品在CG-ECD分析之前进行适当处理，也可以测定亚硝酸根离子和氮的气态氧化物。其他反应物如1,3,5-三甲氧基苯也可用于替代苯。ECD的高灵敏度使得能够对一滴唾液或血液中低至0.1 ppm（w/w）的硝酸盐进行定量。
