Yamada T, Fukuda T, Tamura K, Furukawa S, Songsri P
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Virology. 1993 Dec;197(2):742-50. doi: 10.1006/viro.1993.1650.
A gene encoding a putative translational elongation factor 3 (EF-3) on the genome of Chlorella virus CVK2 has been cloned and sequenced. It encodes for a predicted polypeptide of 1120 amino acids (aa) with a molecular mass of 127 kDa. The overall amino acid sequence of CVK2 EF-3 (vEF-3) showed 36.1% identity and 83.6% similarity to that of Saccharomyces cerevisiae EF-3. Functional domains including two sets of ATP-binding motifs were extremely well conserved between vEF-3 and yeast EF-3; 63.6% identity and 92.4% similarity in total 330-aa portions. Northern blot analysis indicated that the vEF-3 gene was transcribed in the host cells early, at 20 min postinfection (p.i.), as well as late, 3-4 hr p.i. Western blot analyses with anti-vEF-3 antibody detected the 120 kDa vEF-3 protein product after 40 min p.i. It was present until the final stages of infection but absent in the virion. The vEF-3 gene was highly conserved among all Chlorella viruses isolated in Japan.
小球藻病毒CVK2基因组上一个编码假定翻译延伸因子3（EF-3）的基因已被克隆和测序。它编码一个预测的由1120个氨基酸（aa）组成的多肽，分子量为127 kDa。CVK2 EF-3（vEF-3）的整体氨基酸序列与酿酒酵母EF-3的氨基酸序列有36.1%的同一性和83.6%的相似性。包括两组ATP结合基序在内的功能域在vEF-3和酵母EF-3之间极为保守；在总共330个氨基酸的部分中，同一性为63.6%，相似性为92.4%。Northern印迹分析表明，vEF-3基因在宿主细胞感染早期（感染后20分钟）以及晚期（感染后3 - 4小时）转录。用抗vEF-3抗体进行的Western印迹分析在感染后40分钟检测到120 kDa的vEF-3蛋白产物。它一直存在到感染的最后阶段，但在病毒粒子中不存在。vEF-3基因在日本分离的所有小球藻病毒中高度保守。
