Pathak S, Dolhonde J A, Multani A S
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Cytobios. 1998;93(374):141-6.
The distribution of telomeric DNA in the genomes of the antelope ground squirrel, Ammospermophilus harrisii (family Sciuridae; 2n = 32) and the African black-footed cat, Felis nigripes (family Felidae; 2n = 38) were compared by fluorescence in situ hybridization (FISH) technique. These two mammalian species have the highest and the lowest amount of C-banded regions, respectively. FISH preparations with the human telomeric DNA probe showed that all C-banded segments in the A. harrisii chromosomes, except a few intercalary segments, were hybridizing with this DNA. F. nigripes showed hybridization only on the termini of each chromosome, and the C-banded regions did not hybridize with telomeric DNA on FISH analysis. The C-banded chromosomal arms in another rodent species, Peromyscus eremicus (family Cricetidae; 2n = 48), when hybridized with human telomeric DNA showed signals only in the termini of chromosomes but not in the heterochromatic arms. These observations indicate that not all C-banded regions in rodent species are telomeric DNA. The amplification of telomeric DNA in relation to speciation is discussed.
通过荧光原位杂交（FISH）技术比较了羚羊地松鼠（Ammospermophilus harrisii，松鼠科；2n = 32）和非洲黑足猫（Felis nigripes，猫科；2n = 38）基因组中端粒DNA的分布。这两种哺乳动物物种分别具有最高和最低量的C带区域。用人端粒DNA探针进行的FISH制备显示，哈氏羚羊地松鼠染色体中的所有C带片段，除了少数居间片段外，都与该DNA杂交。黑足猫在FISH分析中仅在每条染色体的末端显示杂交信号，并且C带区域不与端粒DNA杂交。另一种啮齿动物物种，荒漠鹿鼠（Peromyscus eremicus，仓鼠科；2n = 48）的C带染色体臂，与人端粒DNA杂交时仅在染色体末端显示信号，而不在异染色质臂中显示信号。这些观察结果表明，啮齿动物物种中的并非所有C带区域都是端粒DNA。讨论了端粒DNA扩增与物种形成的关系。
