抗原剂量反应及淋巴因子嗜酸性粒细胞刺激促进因子产生的特异性
Antigen dose response and specificity of production of the lymphokine eosinophil stimulation promoter.
作者信息
Pelley R P, Karp R, Mahmoud A A, Warren K S
出版信息
J Infect Dis. 1976 Sep;134(3):230-7. doi: 10.1093/infdis/134.3.230.
The present investigations of the recently discovered lymphokine eosinophil stimulation promoter have (1) shown that the assay system is highly sensitive and reproducible via the establishment of dose-response curves and their statistical analysis, (2) demonstrated that the direct assay is specific with use of eosinophilrich peritoneal exudate cells obtained from mice with either schistosomiasis or trichinosis, (3) demonstrated that the indirect test with splenic lymphocytes is specific with use of both the above method and radioisotope-labeled antigens, and (4) provided information on the roles of eosinophils and mononuclear cells via purification, reconstitution, and the use of antiserum to eosinophils.
近期对新发现的淋巴细胞因子嗜酸性粒细胞刺激促进因子的研究表明
（1）通过建立剂量反应曲线并进行统计分析，证明该检测系统高度灵敏且可重复；（2）利用从患有血吸虫病或旋毛虫病的小鼠获得的富含嗜酸性粒细胞的腹腔渗出细胞进行直接检测，证明该检测具有特异性；（3）采用上述方法和放射性同位素标记抗原，利用脾淋巴细胞进行间接检测，证明该检测具有特异性；（4）通过纯化、重组以及使用抗嗜酸性粒细胞抗血清，获取了有关嗜酸性粒细胞和单核细胞作用的信息。
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