Warren K S, Karp R, Pelley R P, Mahmoud A A
J Infect Dis. 1976 Sep;134(3):277-80. doi: 10.1093/infdis/134.3.277.
A test for the lymphokine eosinophil stimulation promoter (ESP) has been adapted to the study of Trichinella spiralis infections in mice and has been used as an aid in the diagnosis of trichinosis in a patient. With use of soluble antigen extracted from T. spiralis larvae and peritoneal exudate cells rich in eosinophils obtained from mice with trichinosis of four weeks' duration, a dose-response curve was constructed and analyzed. The soluble larval antigens greatly enhanced the migration of eosinophils from the mice with trichinosis but had no effect on the eosinophils from mice with schistosomiasis, nor did soluble schistosome egg antigens have any effect on the cells from mice with trichinosis. On two occasions peripheral white blood cells from a patient with presumed T. spiralis infection gave strongly positive results in ESP tests on stimulation with soluble larval antigen, but the cells did not respond significantly to soluble schistosome egg antigen; the result in the ESP test was positive before the patient's serum produced a weakly positive agglutination response.
一种针对淋巴因子嗜酸性粒细胞刺激促进因子（ESP）的检测方法已应用于小鼠旋毛虫感染的研究，并已用于协助诊断一名患者的旋毛虫病。利用从旋毛虫幼虫中提取的可溶性抗原以及从患有四周病程旋毛虫病的小鼠中获取的富含嗜酸性粒细胞的腹腔渗出细胞，构建并分析了剂量反应曲线。可溶性幼虫抗原极大地增强了旋毛虫病小鼠嗜酸性粒细胞的迁移，但对血吸虫病小鼠的嗜酸性粒细胞没有影响，可溶性血吸虫卵抗原对旋毛虫病小鼠的细胞也没有任何影响。有两次，一名疑似感染旋毛虫的患者的外周血白细胞在用可溶性幼虫抗原刺激后的ESP检测中给出了强阳性结果，但这些细胞对可溶性血吸虫卵抗原没有明显反应；在患者血清产生弱阳性凝集反应之前，ESP检测结果即为阳性。
