Jobes D V, Chima S C, Ryschkewitsch C F, Stoner G L
Neurotoxicology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Gen Virol. 1998 Oct;79 ( Pt 10):2491-8. doi: 10.1099/0022-1317-79-10-2491.
The polyomavirus JC (JCV) establishes a persistent infection in the kidneys, and is the virus agent that causes the demyelinating disease progressive multifocal leukoencephalopathy. PCR and DNA sequence analyses of partial JCV genomes have shown that there are at least four main JCV types, each associated with a specific geographical region. Type 1 is of European origin, Type 2 is Asian, Type 3 is found in individuals of African decent and Type 4 is a potential recombinant of Types 1 and 3, and is widely distributed throughout the population of the United States. A comprehensive phylogenetic analysis of 22 complete JCV genomes excluding part of the regulatory region was accomplished using neighbour-joining, UPGMA and maximum parsimony methods. The resulting UPGMA and parsimony phylogenies suggest that the European Type 1 strains diverged from the other types during the evolution of JCV and that each of the other genotypes (and subtypes) falls into well-supported clades. This is the first whole genome approach to phylogeny reconstruction for JCV and represents a significant improvement over earlier studies that were limited to partial JCV sequences and the neighbour-joining method.
多瘤病毒JC（JCV）在肾脏中建立持续性感染，是导致脱髓鞘疾病进行性多灶性白质脑病的病毒病原体。对部分JCV基因组的PCR和DNA序列分析表明，至少有四种主要的JCV类型，每种类型都与特定的地理区域相关。1型起源于欧洲，2型是亚洲型，3型在非洲裔个体中发现，4型是1型和3型的潜在重组体，广泛分布于美国人群中。使用邻接法、UPGMA和最大简约法对22个完整的JCV基因组（不包括部分调控区域）进行了全面的系统发育分析。由此得到的UPGMA和简约系统发育树表明，欧洲1型毒株在JCV进化过程中与其他类型发生了分化，其他每个基因型（和亚型）都落入了支持良好的分支中。这是首次采用全基因组方法对JCV进行系统发育重建，相较于早期仅限于部分JCV序列和邻接法的研究有了显著改进。
