Agostini H T, Shishido-Hara Y, Baumhefner R W, Singer E J, Ryschkewitsch C F, Stoner G L
Neurotoxicology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4126, USA.
J Gen Virol. 1998 May;79 ( Pt 5):1143-51. doi: 10.1099/0022-1317-79-5-1143.
Five major genotypes of JC virus (JCV) have been defined based on nucleotide differences in the VP1 gene of the DNA sequence. These types are probably a result of virus evolution in geographically isolated population groups. One of the first genotypes identified, Type 2, was found to represent strains of Asian origin. In order to further define the spectrum within Type 2 strains, the entire 5.1 kb genome of nine urinary strains of JCV was amplified by PCR with one pair of primers. These urine samples were obtained in the USA (California and New Mexico) from three European Americans, three Native Americans, two African Americans and one Hispanic American. The complete genome of an Asian JCV strain (Tokyo-1) isolated from progressive multifocal leukoencephalopathy (PML) brain in Japan was also sequenced. Here, we report the analysis of these ten DNA sequences and their deduced protein translations. Two phylogenetically distinct subtypes of Type 2 were found, 2A and 2B, which differ from each other by 0.8-1.1% of the coding region sequence. A 215 bp product amplified with primers in the VP1 gene contains enough sequence information to distinguish the major types and subtypes of JCV and is suitable for application in viral epidemiological studies. The investigation of these genomic variations is of special interest because JCV Type 2 strains are found at a significantly higher frequency in brain tissue of patients with PML than would be predicted from their excretion in a control population.
基于DNA序列中VP1基因的核苷酸差异，已确定了JC病毒（JCV）的五种主要基因型。这些类型可能是病毒在地理上隔离的人群组中进化的结果。最早确定的基因型之一，即2型，被发现代表亚洲起源的毒株。为了进一步确定2型毒株的谱系，用一对引物通过PCR扩增了9株JCV尿液毒株的整个5.1 kb基因组。这些尿液样本取自美国（加利福尼亚州和新墨西哥州）的三名欧裔美国人、三名美洲原住民、两名非裔美国人和一名西班牙裔美国人。还对从日本进行性多灶性白质脑病（PML）脑部分离出的一株亚洲JCV毒株（东京-1）的完整基因组进行了测序。在此，我们报告对这十个DNA序列及其推导的蛋白质翻译的分析。发现2型有两个系统发育上不同的亚型，2A和2B，它们在编码区序列上彼此相差0.8 - 1.1%。用VP1基因中的引物扩增出的一个215 bp产物包含足以区分JCV主要类型和亚型的序列信息，适用于病毒流行病学研究。对这些基因组变异的研究具有特殊意义，因为在PML患者的脑组织中发现2型JCV毒株的频率明显高于根据其在对照人群中的排泄情况所预测的频率。
